Kanamycin
Cat.No：K8020 Solarbio
CAS：25389-94-0
Appearance：White Powder
Storage：Store at 2-8℃，4 years
Purity：potency(Anhybrous)(mcg/mg)≥750
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
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ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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MAPK
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Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Kanamycin |
| CAS | 25389-94-0 |
| Molecular Formula | C18H36N4O11·H2SO4 |
| Molecular Weight | 582.58 |
| Storage | Store at 2-8℃，4 years |
| Appearance | White Powder |
| Purity | potency(Anhybrous)(mcg/mg)≥750 |
| Grade | USP Grade |
| Solubility | 100mg/mL in Water |
| Hazard Code | R：36/37/38；60/61；42/43 S：36/37/39 |
| Unit | Bottle |
| Specification | 1g 5g 10g 25g 100g |
Product Introduction:
Kanamycin sulfate is an aminoglycoside antibiotic that inhibits both gram-positive and Gram-negative bacteria and mycoplasma. The mechanism of action is to target binding to the bacterial 70S ribosome subunit, thereby inhibiting ribosome translocation and causing miscoding to further interfere with protein synthesis.
Description:
Kanamycin sulfate is an aminoglycoside antibiotic that binds to the 70S ribosomal subunit to inhibit peptide chain formation and is used for resistance screening.
Use and synthesis method:
Kanamycin sulfate can be used to inhibit bacterial contamination in cell culture. In molecular biology studies, it is commonly used to select bacterial clones that successfully transform kanamycin resistance gene (kan gene). It is also commonly used in Agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying the npt II (APH3) gene. The product is soluble in water (50 mg/ml) and should be used after filtration and sterilization. The recommended working concentration is 30-100μg/ml.
Physical shape:
Moisture (%) : ≤4.0
pH (1%, water) 25℃ : 6.5-8.5
Ash (%) : ≤1.0
Solubility:
Soluble in water, 10-50mg/ml as mother liquor, stored at 2-8 ℃
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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该抗生素用于选择培养基（添加琼脂）时，对温度有何要求（70摄氏度能否添加） 2023-07-20 21:56:32
Administrator您好，在培养基中添加硫酸卡那霉素时，需要培养基不烫手时添加，以防温度过高抗生素失效