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My Cart> Antibodies > Secondary Antibodies > Purified Secondary Antibodies > Rabbit anti-Human IgGReactivity：Human
Application： WB,ELISA
Appearance：Liquid
Storage：Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Host：Rabbit
Qty：
Size：
| Name | Rabbit Anti-Human IgG（Purified Antibody） |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-20000.;ELISA:1:2000-100000. |
| Background | Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Immunogen | Human IgG full length protein |
| Calculated MW | 150kDa |
| Purification Method | Affinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
| Appearance | Liquid |
| Concentration | 10mg/mL |
| Piece | Piece |
| Y-Application | ELISA WB |
| Y-Dilution Ratio | WB 1:1000-20000.;ELISA:1:2000-100000. |
| Specification | 1mg 10mg 100mg |
| Reactivity | Human |
| Application | WB ELISA |
WB=1:1000-10000 ELISA=1:1000-10000 IHC-P=1:100-1000 IHC-F=1:100-1000 IF=1:100-1000
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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