GST Fusion Protein Purification Beads

GST fusion protein purification magnetic bead is a new functional material designed for efficient and rapid purification of glutathione S-transferase (GST) fusion protein, which can be directly purified from biological samples in one step by magnetic separation, greatly simplifying the purification process and improving the purification efficiency. It is suitable for convenient purification of GST fusion protein in scientific and industrial fields.

Compared with the traditional column chromatography purification method, the purification of GST fusion protein by magnetic beads does not require multiple long-term high-speed centrifugation of crude protein samples, nor does it require filtration operations. There is no need to control the flow rate and no need for expensive chromatographic equipment. The specific binding of the sample to the magnetic bead, washing and elution of the target protein become very simple, fast and easy to operate, there is no bottleneck of sample flow rate control, and for skilled operators, high purity of the target protein can be obtained within one hour, which not only simplifies the process for researchers, saves time, reduces costs, and improves the purification efficiency of the target protein. The use of magnetic beads can easily achieve multi-sample parallel processing, achieve high throughput, and can also be arbitrarily enlarged sample purification scale.

Application example

1. Comparison of purification of GST fusion protein by magnetic beads and traditional chromatographic column

2%, 5%, 10%, 20% and 100% in the figure refer to the elution of magnetic beads after adsorption of target proteins with buffers containing 0.2mM, 0.5mM, 1mM and 10mM, respectively.

2. Parallel operation has high stability and can facilitate high throughput and large-scale protein purification

Results: CV(target protein purity) = 1.6%, CV(target protein load) = 4.5%.

In addition, when scaling up the sample purification, only the amount of magnetic beads can be increased in the same proportion, which does not affect the yield and purity of the target protein, and there is no restriction on the flow rate and sample volume, which is more suitable for large-scale protein purification production.

3. The product performance between different batches is stable, and the difference between batches is small

Product features

1. Fast protein purification

The operation is simple, one step purification can obtain high purity protein;

Convenient and fast, one hour easy to achieve protein purification.

2. Flexible protein purification

Can process multiple samples at the same time to achieve high throughput;

The protein concentration and volume can be easily controlled to facilitate subsequent sample refining;

Control of purification scale can be easily achieved;

has good purification effect on trace and low abundance samples.

3. Economy of protein purification

Short purification process, high protein production and activity;

The equipment is simple, reducing the cost of equipment purchase and maintenance, and the threshold of protein purification is greatly reduced;

Purification time is short, saving time cost;

Multi-sample processing can be carried out at the same time to realize automatic operation and save labor costs;

Can be reused.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.