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My CartCAS：154-42-7
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to yellow Solid
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| CAS | 154-42-7 |
| Name | 6-Thioguanine |
| Molecular Formula | C5H5N5S |
| Molecular Weight | 167.19 |
| Solubility | Soluble in DMSO |
| Purity | ≥98% |
| Appearance | White to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 205-827-2 |
| MDL | MFCD00233553 |
| SMILES | SC1=NC(N)=NC2=C1N=CN2 |
| InChIKey | WYWHKKSPHMUBEB-UHFFFAOYSA-N |
| InChI | InChI=1S/C5H5N5S/c6-5-9-3-2(4(11)10-5)7-1-8-3/h1H,(H4,6,7,8,9,10,11) |
| PubChem CID | 2723601 |
| Target Point | Influenza Virus(SARS-CoV) |
| Passage | Anti-infection |
| Background | 6-Thioguanine is an immunosuppressant and an inhibitor of SARS and MERS coronavirus papain-like proteases (PLpros), and can effectively inhibit the activity of USP2. Can be used for anti-leukemia related research. |
| Biological Activity | 6-Thioguanine 是一种抗白血病和免疫抑制剂, 为 SARS 和 MERS 冠状病毒木瓜样蛋白酶 (PLpros) 的抑制剂, 同时可有效抑制 USP2 的活性, IC50 值分别为 25 μM 和 40 μM[3]。 |
| IC50 | IC50: 25μM (PLpros) ， 40μM (Recombinant human USP2) [3] |
| In Vitro | 6-Thioguanine是一种抗白血病和免疫抑制剂，可作为SARS和MERS冠状病毒木瓜蛋白酶样蛋白酶(PLpros)的抑制剂，并有效抑制USP2活性，对于Plpros和重组人USP2，IC50为25μM和40μM，分别[1]。 6-硫鸟嘌呤通过纯化的DNA甲基转移酶(包括人DNMT1和细菌HpaII甲基化酶)影响胞嘧啶残基的甲基化。 6-Thioguanine(1或3μM)降低Jurkat T细胞中的全局胞嘧啶甲基化和3μM人细胞中的胞嘧啶甲基化[2]。 6-硫鸟嘌呤(18.75，37.50或75.00μM)对细胞活力产生不利影响，但对LDH或ALT活性没有影响[3]。 |
| Cell Experiment | 治疗由6种浓度(0.468，0.937，1.875，3.750，7.500和15.000μM)中的3种硫代嘌呤(硫唑嘌呤，6-巯基嘌呤和6-硫鸟嘌呤)组成。将每种硫嘌呤溶解在DMSO溶液中以达到10mg / mL的浓度。使用无菌过滤的维持培养基将每种硫嘌呤溶液进一步稀释至6种处理浓度中的每一种。将肝细胞接种于96孔培养物后24小时[3]。 |
| Data Literature Source | [1]. Chuang SJ， et al. 6-Thioguanine is a noncompetitive and slow binding inhibitor of human deubiquitinating protease USP2. Sci Rep. 2018 Feb 15; 8 (1) :3102. [2]. Wang H， et al. 6-Thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo. Biochemistry. 2009 Mar 17; 48 (10) :2290-9. [3]. LaDuke KE， et al. Effects of azathioprine， 6-mercaptopurine， and 6-thioguanine on canine primary hepatocytes. Am J Vet Res. 2015 Jul; 76 (7) :649-55 |
| Unit | Bottle |
| Specification | 50mg 10mM*1mL in DMSO 100mg |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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