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My CartCAS：18883-66-4
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 18883-66-4 |
| Name | Streptozocin |
| Molecular Formula | C8H15N3O7 |
| Molecular Weight | 265.22 |
| Solubility | Soluble in Water/DMSO/Sodium Citrate buffer(0.1mol/L, pH4.5, sterile)（The product is unstable after dissolution. It is recommended that you use it immediately after dissolution.） |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 242-646-8 |
| MDL | MFCD00006607 |
| SMILES | O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](NC(N(C)N=O)=O)[C@H]1O |
| InChIKey | ZSJLQEPLLKMAKR-GKHCUFPYSA-N |
| InChI | InChI=1S/C8H15N3O7/c1-11(10-17)8(16)9-4-6(14)5(13)3(2-12)18-7(4)15/h3-7,12-15H,2H2,1H3,(H,9,16)/t3-,4-,5-,6-,7+/m1/s1 |
| PubChem CID | 29327 |
| Target Point | DNA Methylation |
| Passage | Epigenetics |
| Background | Streptozocin is a potent DNA methylation agent. STZ can increase the blood sugar of mice, and is a commonly used inducer of diabetes modeling. Meanwhile, the diabetic effect of STZ is different in species. This product is unstable, it is recommended to use now.） |
| Biological Activity | Streptozocin是一种有效的 DNA甲基化试剂，在HL60，K562和C1498细胞中的IC50 分别为11.7，904 和 1024 μg/mL。还是一种糖尿病诱导剂，可诱导自噬与凋亡。[1-3] |
| In Vitro | 与四氧嘧啶(ALX)相比，链脲佐菌素(STZ)在体外对血液细胞系显示出更高的细胞毒性作用。对于研究的细胞系，ALX似乎没有毒性，HL60，K562和C1498细胞的IC50值估计分别为2809，3679或超过4000μg/ mL。链脲佐菌素毒性更大，特别是对人类髓系白血病细胞系HL60。对于HL60，K562和C1498细胞，链脲佐菌素的IC50值分别为11.7，904和1024μg/ mL。结果还表明，鼠白血病细胞对链脲佐菌素和ALX细胞毒性的耐药性高于人白血病细胞[2]。 |
| In Vivo | 注射链脲佐菌素(STZ)的小鼠显示出比注射ALX的动物体重低的倾向。与注射ALX的小鼠(60.7±4.3×106; n = 15; p = 0.01)相比，注射链脲佐菌素的小鼠脾细胞明显减少(22.2±3.2×106; n = 10)[2]。 |
| Cell Experiment | 在不存在(未处理的对照)或存在各种浓度的ALX(20-3000μg/ mL)或STZ(1)的情况下，人和鼠细胞系在96孔板中以2×10 4细胞/孔的密度一式三份培养。 -3000μg/ mL)在37℃，含5％CO 2的潮湿气氛下48小时。在包括终浓度为0.1％的dH 2 O的完全培养基中孵育的细胞用作溶剂毒性的对照，并且在完全培养基中孵育的细胞用作实验的对照。使用MTT测定法根据制造商的说明书测定测试药物对肿瘤细胞生长或活力的影响。使用GraphPad Prism 4程序计算IC 50值(诱导50％细胞生长抑制的药物浓度)[2]。 |
| Animal Experiment | 小鼠[2]使用雄性C57BL / 6小鼠(10-16周)。用链脲佐菌素和ALX以及对照处理的小鼠组中的年龄组分布如下：链脲佐菌素异种移植物(n = 7，中位年龄14周))，ALX异种移植(n = 11，中位年龄15周)，链脲霉素非移植(n = 7，中位年龄14周)，ALX未移植(n = 15，中位年龄15周)。男性C57BL / 6小鼠通过阴茎静脉注射ALX(75 mg / mL)或链脲佐菌素(180 mg / kg)进行吸入麻醉。对照组含有雄性C57BL / 6小鼠。在注射前，6小时后，然后在药物注射后每天测量血糖水平和体重。大鼠[3] 30只大鼠接受卵巢切除术以诱导绝经状态。使用腹膜内注射氯胺酮盐酸盐(90mg / kg)和甲苯噻嗪(10mg / kg)，在麻醉后通过中线切口在无菌条件下进行卵巢切除术。用3.0多聚半乳糖缝合线封闭切口。剩余的10只大鼠形成对照组(组1)。接受卵巢切除术的30只大鼠中有10只构成了更年期对照组(第2组)。在手术后1周开始，每天注射甲基强的松龙(1mg / kg)直至研究结束，以模拟10只大鼠(第3组)的类风湿性疾病治疗风湿性疾病。第4组由在卵巢切除术后1周接受腹膜内施用链脲佐菌素(50mg / kg)以诱导糖尿病(DM)的大鼠组成。在施用链脲佐菌素后3天检查血糖水平，并且> 250mg / dL的值被认为是DM的阳性。 |
| Data Literature Source | [1]. Bennett RA， et al. Alkylation of DNA in rat tissues following administration of streptozotocin. Cancer Res. 1981 Jul; 41 (7) :2786-90 [2]. Diab RA， et al. Immunotoxicological effects of streptozotocin and alloxan: in vitro and in vivo studies. Immunol Lett. 2015 Feb; 163 (2) :193-8 [3]. Acer S， et al. Oxidative stress of crystalline lens in rat menopausal model. Arq Bras Oftalmol. 2016 Jul-Aug; 79 (4) :222-5 |
| Unit | Bottle |
| Specification | 10mg 20mg 100mg 500mg |
Examples of using this product（for reference only）
In Vivo:
Rats（male Sprague?Dawley rats，250?300 g，10 weeks old；65 mg/kg Streptozocin；I.P）:
A total of 20 male Sprague?Dawley rats (250?300 g, 10 weeks old) were fasted for 12 h and intraperitoneally injected with streptozotocin (65 mg/kg) to induce type I diabetes mellitus. Rats with blood glucose level above 16.7 mmol/L under normal conditions were regarded as successful diabetic animal models and were utilized for surgery.
Reference：
Hao Z, Liu G, Ren L, Liu J, Liu C, Yang T, Wu X, Zhang X, Yang L, Xia J, Li W. A Self-Healing Multifunctional Hydrogel System Accelerates Diabetic Wound Healing through Orchestrating Immunoinflammatory Microenvironment. ACS Appl Mater Interfaces. 2023 Apr 26;15(16):19847-19862. doi: 10.1021/acsami.2c23323. Epub 2023 Apr 12. PMID: 37042619.
Rats（Wistar rats；25 mg/kg Streptozotocin；i. p.）:
In some experiments, high fat diet - streptozotocin (HFD-STZ) diabetic rat were used. To this end, 4-week-old Wistar rats were fed on a 60% high fat diet (HFD) for 8 weeks and then received 25 mg/kg i. p. streptozotocin (Solarbio, China). Their blood was sampled at 13 weeks of age.
Reference：
Zhen D, Ding L, Wang B, Wang X, Hou Y, Ding W, Portha B, Liu J. Oral administration of kynurenic acid delays the onset of type 2 diabetes in Goto-Kakizaki rats. Heliyon. 2023 Jun 27;9(7):e17733. doi: 10.1016/j.heliyon.2023.e17733. PMID: 37424591; PMCID: PMC10328841.
Mice （Male ICR mice (6-week old, 30±2 g) ，35mg/kg STZ，I.P， 1/week，total 4 times）:
After one week of acclimation, mice were fed with a 60% high-fat diet for hyperglycemic model induction. After 9 weeks, the HFD-fed mice were intraperitoneally injected with STZ (35 mg/kg) once a week for 4 times. One week later, the fasting blood glucose level (FBG) was measured after 12 h fasting, and the mice with FBG > 8 mM were considered as hyperglycemic model mice. Subsequently, the model group was divided into control and Up-U groups. The Up-U group was orally administered with Up-U at a dose of 200 mg/kg/day for 12 weeks. The control group was orally administered with the same amount of sterile water and all hyperglycemic model mice were fed with a 60% high-fat diet. The mice fed with a chow diet during the whole experiment were used as the blank group. The FBG levels of the mice were measured after 12 h fasting for every 4 weeks.
Reference：
Zhong QW, Zhou TS, Qiu WH, Wang YK, Xu QL, Ke SZ, Wang SJ, Jin WH, Chen JW, Zhang HW, Wei B, Wang H. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida. Food Chem. 2021 Mar 30;341(Pt 1):128148. doi: 10.1016/j.foodchem.2020.128148. Epub 2020 Sep 22. PMID: 33038776.
Mice（Male C57BL/6 J mice；55 mg/kg Streptozotocin, I.P, 3d）:
Male C57BL/6 J mice (Eight- to ten-week-old) were kept under specific pathogen-free conditions. After a fast of 12 h, mice received intraperitoneal injection of 55 mg/kg/d streptozotocin (Solarbio, Beijing, China) or equal volume of citrate buffer (0.1 M, pH = 4.5) respectively for 3 d. Mice with a fasting plasma glucose level > 16 mmol/L were chosen to use in this study. Mice were randomly divided into 3 groups: sham group, MCAO/R group and MCAO/ R + Dex group. Mice in the MCAO/R + Dex group were injected in_x005f traperitoneally with Dex (25 μg/kg) after MCAO/R surgery.
Reference：
Chen L, Cao J, Cao D, Wang M, Xiang H, Yang Y, Ying T, Cong H. Protective effect of dexmedetomidine against diabetic hyperglycemia-exacerbated cerebral ischemia/reperfusion injury: An In Vivo and In Vitro study. Life Sci. 2019 Oct 15;235:116553. doi: 10.1016/j.lfs.2019.116553. Epub 2019 Jun 8. PMID: 31185237.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Zhong QW, et al. Food Chem. 2021 Mar 30;341(Pt 1):128148.
Zhen D, et al. Heliyon. 2023 Jun 27;9(7):e17733.
Manual Download
STZ可以配制母液长期保存吗？具体的母液配制方法如何？ 2023-10-07 13:23:18
Administrator母液保存在-20℃, 有效期1个月。配置好的母液建议按照实验需要分装冻存，避免反复冻融。