Sanguinarine
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Cat.No：IS0030 Solarbio
CAS：2447-54-3
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Solid
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> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Apoptosis > Sanguinarine
Product Information
| CAS | 2447-54-3 |
| Name | Sanguinarine |
| Molecular Formula | C20H14NO4 |
| Molecular Weight | 332.33 |
| Solubility | Soluble in DMSO |
| Purity | HPLC≥98% |
| Appearance | Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 219-503-3 |
| MDL | MFCD00064925 |
| SMILES | C[N+]1=CC2=C3C(OCO3)=CC=C2C(C=CC4=C5)=C1C4=CC6=C5OCO6 |
| InChIKey | INVGWHRKADIJHF-UHFFFAOYSA-N |
| InChI | InChI=1S/C20H14NO4/c1-21-8-15-12(4-5-16-20(15)25-10-22-16)13-3-2-11-6-17-18(24-9-23-17)7-14(11)19(13)21/h2-8H,9-10H2,1H3/q+1 |
| PubChem CID | 5154 |
| Target Point | Others |
| Passage | Others |
| Background | Sanguinarine is an alkaloid that can stimulate cell apoptosis by activating the production of reactive oxygen species (ROS). Sanguinarine induced apoptosis and JNK and NF- κ B activation. |
| Biological Activity | Sanguinarine 是一种来源于 Sanguinaria Canadensis 的生物碱，可通过激活活性氧 (ROS) 的产生来刺激细胞凋亡。Sanguinarine 诱导的细胞凋亡与 JNK 和 NF-κB 的活化有关。[1] |
| In Vitro | Sanguinarine(SANG)诱导的细胞凋亡与JNK和NF-κB信号通路的激活有关。为了确定Sanguinarine对细胞活力的影响，用不同浓度的Sanguinarine刺激22B-cFluc细胞24小时，然后用CKK刺激细胞凋亡。进行-8测定。 Sanguinarine治疗以剂量依赖性方式降低22B细胞的增殖。同时，测量用不同剂量的血根碱处理的22B-cFluc细胞的胞质提取物，以使用Ac-DEVD-pNA检测细胞半胱天冬酶-3活性，所述Ac-DEVD-pNA是经验证的半胱天冬酶-3底物。 450 nm处的吸光度以剂量依赖性方式增加，表明Sanguinarine刺激的caspase-3活性增加[1]。 |
| In Vivo | 为了评估Sanguinarine(SANG)在体内诱导的细胞凋亡，将22B-cFluc细胞皮下接种到裸鼠的一侧，并允许异种移植模型建立。用10mg/kg血根碱静脉内治疗小鼠。在处理后24，48和72小时，在用150mg/kg D-荧光素底物ip注射小鼠后进行生物发光成像。 Sanguinarine治疗早在初始治疗后48小时就会引起明显的发光信号增加。在整个实验过程中观察到持续的生物发光成像(BLI)强度增加。在处理后72小时，收集肿瘤并进行TUNEL染色以评估细胞凋亡。与对照肿瘤相比，Sanguinarine治疗组表现出明显更多的细胞凋亡，表明来自散发性凋亡细胞的绿色信号增加[1]。 |
| Cell Experiment | 使用细胞计数试剂盒-8通过CCK-8测定法测定血根碱的细胞活力。简而言之，将22B-cFluc细胞接种在96孔板(5×103细胞/孔)中，并用不同浓度的血根碱(0.5μM，1μM，2μM，4μM)处理24小时。然后向每个孔中加入10mL CKK-8，持续4小时，用酶标仪测量450nm处的吸光度。确定光密度(OD)值以反映来自每个孔的活细胞群[1]。 |
| Animal Experiment | 小鼠[1]通过将悬浮在PBS中的1×10 7个22B-cFluc细胞注射到裸鼠(n = 6)中来制备异种移植肿瘤模型。在肿瘤达到约100mm 3的体积后，将血根碱(10mg/kg)静脉内注射到小鼠中。注射24小时，48小时和72小时后，给小鼠单剂量150mg/kg D-荧光素，并使用Xenogen Lumina II系统进行生物发光成像。使用Living Image软件4.1表示感兴趣区域中的信号强度。对于抗肿瘤治疗研究，一组荷瘤小鼠(n = 6)在整个实验期间每隔一天静脉注射10mg/kg血根碱，而对照组小鼠(n = 6)仅接受DMSO 。计算肿瘤生长测量[1]。 |
| Kinase Experiment | 使用胱天蛋白酶-3活性测定试剂盒测量胱天蛋白酶-3活性。简而言之，收集用不同浓度的血根碱(0.5μM，1μM，2μM，4μM)或对照DMSO处理的细胞，洗涤并在裂解缓冲液中在冰上裂解30分钟。然后通过以1，2000rpm离心10分钟收集上清液。向每个样品中加入Ac-DEVD-pNA(2mM)并在37℃下孵育1小时。最后使用分光光度计在405nm的波长下量化每个样品的光密度(OD)。 p-NA标准用于校准每个样品的caspase-3活性[1]。 |
| Data Literature Source | [1]. Wang Y，Noninvasive bioluminescence imaging of the dynamics of sanguinarine induced apoptosis via activation of reactive oxygen species. Oncotarget. 2016 Apr 19;7(16):22355-67. |
| Unit | Bottle |
| Specification | 5mg 10mg |
是一种生物碱，可通过激活活性氧 (ROS) 的产生来刺激细胞凋亡。诱导的细胞凋亡与 JNK 和 NF-κB 的活化有关。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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