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My CartCAS：10338-51-9
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 10338-51-9 |
| Name | Salidroside |
| Molecular Formula | C14H20O7 |
| Molecular Weight | 300.3 |
| Solubility | Soluble in Water/Ethanol ≥10mg/mL;Soluble in DMSO ≥ 3mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 695-621-2 |
| MDL | MFCD00210553 |
| SMILES | O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1OCCC2=CC=C(O)C=C2 |
| InChIKey | ILRCGYURZSFMEG-RKQHYHRCSA-N |
| InChI | InChI=1S/C14H20O7/c15-7-10-11(17)12(18)13(19)14(21-10)20-6-5-8-1-3-9(16)4-2-8/h1-4,10-19H,5-7H2/t10-,11-,12+,13-,14-/m1/s1 |
| PubChem CID | 159278 |
| Target Point | prolyl endopeptidase |
| Passage | PI3K/Akt/mTOR;Metabolic Enzyme&Protease |
| Background | It is a prolyl endopeptidase inhibitor. |
| Biological Activity | Salidroside 是一种脯氨酰内肽酶 (prolyl endopeptidase) 抑制剂。Salidroside 可通过激活 mTOR 信号缓解肿瘤恶病质小鼠模型中的恶病质症状。[1-4] |
| In Vitro | 红景天苷(100μM)抑制脯氨酰内肽酶(PEP)活性(10.6±1.9％)。脯氨酰内肽酶是一种在含有脯氨酸的神经肽酶的代谢中起作用的酶，其被认为参与学习和记忆[1]。红景天甙是在R. rosea L中发现的主要苯丙烷苷之一，在许多国家几乎每天作为营养补充剂食用，并且已被确定具有潜在的抗疲劳和缺氧，抗衰老和抗阿尔茨海默病活动。红景天甙可通过增加mTOR，p-mTOR和MyHC表达来改善肌肉营养[2]。将SH-SY5Y细胞暴露于0-600μMMPP+ 12-48小时，结果显示MPP +以浓度和时间依赖性方式导致细胞活力显著降低。将细胞用25-100μM红景天苷(Sal)预处理24小时，然后暴露于500μMMPP+另外24小时。红景天苷浓度依赖性地阻止MPP +诱导的细胞活力降低。膜联蛋白V/PI染色是检测凋亡细胞的常用方法。红景天甙显著降低MPP +处理的膜联蛋白V/PI染色细胞的数量，其浓度依赖性。凋亡细胞也可以通过Hoechst染色进行形态学评估。在Hoechst染色中，凋亡细胞的特征在于核大小减小，染色质浓缩，强荧光和核碎裂。红景天甙显著抑制MPP +诱导的SH-SY5Y细胞中染色质浓缩，强荧光和核碎裂的增加[3]。 |
| In Vivo | 红景天甙是从药用植物红景天提取的天然抗氧化剂。红景天甙(100mg/kg /天)对db/db小鼠显示出强烈的葡萄糖降低作用，其类似于二甲双胍(200mg/kg /天)的作用。因此，使用剂量为100 mg/kg /天的红景天苷[4]。 |
| Cell Experiment | 将SH-SY5Y细胞以每孔1×10 4个细胞接种于96孔板中。在用红景天苷(25-100μM)和MPP +处理后，通过MTT测定法测量细胞活力。简言之，将细胞与500μg/ mL MTT在37℃下孵育4小时。之后，除去培养基并加入150μLDMSO并进行振荡10分钟。在酶标仪中在570nm处测量吸光度，结果表示为对照的倍数[3]。 |
| Animal Experiment | 小鼠[4]给4周龄雄性C57BL/6小鼠喂食高脂肪饮食(HFD)(n = 16)或正常饲料(n = 8)。在HFD 10周后，通过管饲法每天一次开始红景天苷干预(100mg/kg /天)，持续5周。对照组给予载体(盐水)。使用4周龄雄性C57B1/KsJ(BKS)小鼠(野生型，n = 8)和BKS.Cg-Dock7m +/+ Leprdb/J(db/db)小鼠(n = 16)。红景天苷(100mg/kg /天)通过强饲法口服给药，每天一次，持续5周。对照组给予载体(盐水)。每5天监测小鼠的空腹血糖和体重。使用Glucometer对从尾静脉抽取的血液进行葡萄糖测量。 |
| Data Literature Source | [1]. Fan W，et al. Prolyl endopeptidase inhibitors from the underground part of Rhodiola sachalinensis. Chem Pharm Bull (Tokyo). 2001 Apr;49(4):396-401. [2]. Chen X，et al. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activatingmTOR signalling. J Cachexia Sarcopenia Muscle. 2016 May;7(2):225-32. [3]. Wu L，et al. Salidroside Protects against MPP+-Induced Neuronal Injury through DJ-1-Nrf2 Antioxidant Pathway. Evid Based Complement Alternat Med. 2017;2017:5398542. [4]. Ju L，et al. Salidroside，A Natural Antioxidant，Improves β-Cell Survival and Function via Activating AMPK Pathway. Front Pharmacol. 2017 Oct 18;8:749. |
| Unit | Bottle |
| Specification | 5mg 10mM*1mL in DMSO 10mM*1mL in Water 10mg 50mg |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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10mM*1mL in Water 50mg这个一共是50毫克里面是用水溶的红景天苷里面的浓度是10mM*1mL噻 2023-07-20 11:25:41
Administrator您好，10mM*1mL in Water是指红景天苷浓度是10mM,溶剂是水，体积是1ml