Oxaliplatin
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Cat.No：IO0100 Solarbio
CAS：61825-94-3
Storage：Powder:2-8℃，2 years
Purity：HPLC≥98%
Appearance：White to off-white Solid
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> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Cell Cycle > Oxaliplatin
Product Information
| CAS | 61825-94-3 |
| Name | Oxaliplatin |
| Molecular Formula | C8H14N2O4Pt |
| Molecular Weight | 397.29 |
| Solubility | Soluble in DMF ≥4mg/mL(* This product is not stable in solution, it is recommended that you use it now) |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years |
| EC | EINECS 621-248-1 |
| MDL | MFCD00866327 |
| SMILES | O=C1[O-][Pt+2]2([NH2][C@@]3([H])[C@](CCCC3)([H])[NH2]2)[O-]C1=O |
| InChIKey | DRMCATBEKSVAPL-BNTLRKBRSA-N |
| InChI | InChI=1S/C6H12N2.C2H2O4.Pt/c7-5-3-1-2-4-6(5)8;3-1(4)2(5)6;/h5-8H,1-4H2;(H,3,4)(H,5,6);/q-2;;+2/t5-,6-;;/m1../s1 |
| PubChem CID | 9887053 |
| Target Point | Autophagy;DNA/RNA Synthesis |
| Passage | Autophagy;Cell Cycle;DNA Damage/DNA Repair |
| Background | Oxaliplatin is a DNA synthesis inhibitor that causes DNA cross-linking damage， prevents DNA replication and transcription and leads to cell death， which can induce autophagy. |
| Biological Activity | Oxaliplatin是一种 DNA 合成 抑制剂。 它会导致DNA交联损伤，阻止DNA复制和转录并导致细胞死亡。[1-7] |
| In Vitro | 奥沙利铂诱导原发性和继发性DNA损伤导致细胞凋亡[1]。奥沙利铂抑制人黑色素瘤细胞系C32和G361，IC50值分别为0.98 mM和0.14 mM [2]。奥沙利铂有效抑制膀胱癌细胞系RT4和TCCSUP，卵巢癌细胞系A2780，结肠癌细胞系HT-29，胶质母细胞瘤细胞系U-373MG和U-87MG，以及黑素瘤细胞系SK-MEL-2和HT-144 IC50分别为11μM，15μM，0.17μM，0.97μM，2.95μM，17.6μM，30.9μM和7.85μM[3]。 |
| In Vivo | 奥沙利铂(10 mg/kg，ip)显著降低携带肝细胞HCCLM3肿瘤的裸鼠的肿瘤体积和凋亡指数[4]。奥沙利铂(5mg/kg，iv)对T-白血病 - 淋巴瘤L40 AKR有效，T/C为1.77。奥沙利铂对脑内移植的L1210白血病，MA16-C异种移植物，B16黑素瘤异种移植物，Lewis肺异种移植物和C26结肠癌异种移植物有效[5]。奥沙利铂诱导小鼠逆行神经元转运受损[6]。 |
| Cell Experiment | 通常，在第0天将细胞接种到96孔板中，并在第1天暴露于奥沙利铂;在奥沙利铂暴露后48小时进行磺酰罗丹明-B测定。除非添加奥沙利铂和在最终测定期间，否则将板在37℃，5％CO 2和100％相对湿度下一直温育。用于测定的初始细胞数范围为2-20×10 3个细胞/ 50/nL /孔。用于平板接种的细胞数和药物暴露时间基于试验研究，使用以下标准：(a)对照孔中的细胞在测定当天仍处于生长的对数期;(b)测定当天未处理对照的最大吸光度范围为1.0至1.5;(c)在药物暴露期间细胞经历> 2次加倍。每个浓度使用8个孔。在570和/或540nm处读板。 |
| Animal Experiment | HCCLM3产生的HCC肿瘤模型通过在0.2mL无血清培养基中皮下注射5×105 HCCLM3细胞到左上腹部区域而在裸鼠中建立。三天后，将小鼠随机分配接受以下三种治疗中的一种：i)每周腹膜内(ip)注射蒸馏水(对照组，n = 8); ii)每周ip注射5mg/kg的奥沙利铂(低剂量组，n = 7);或iii)每周ip注射10mg/kg的奥沙利铂(高剂量组，n = 7)。通过每5天用卡尺测量每个肿瘤的两个二等分直径来监测肿瘤生长。使用公式(V = a×b2/2)计算肿瘤体积，a为较大直径，b为较小直径。在奥沙利铂给药后第32天对小鼠实施安乐死。 |
| Data Literature Source | [1]. Raymond E，et al. Oxaliplatin: a review of preclinical and clinical studies. Ann Oncol. 1998 Oct;9(10):1053-71. [2]. Mohammed MQ，et al. Oxaliplatin is active in vitro against human melanoma cell lines: comparison with cisplatin and carboplatin. Anticancer Drugs. 2000 Nov;11(10):859-63. [3]. Pendyala L，et al. In vitro cytotoxicity，protein binding，red blood cell partitioning，and biotransformation of oxaliplatin. Cancer Res. 1993 Dec 15;53(24):5970-6. [4]. Wang Z，et al. Oxaliplatin induces apoptosis in hepatocellular carcinoma cells and inhibits tumor growth. Expert Opin Investig Drugs. 2009 Nov;18(11):1595-604 [5]. Mathé G，et al. Oxalato-platinum or 1-OHP，a third-generation platinum complex: an experimental and clinical appraisal and preliminary comparison with cis-platinum and carboplatinum. Biomed Pharmacother. 1989;43(4):237-50. [6]. Schellingerhout D，et al. Impairment of retrograde neuronal transport in oxaliplatin-induced neuropathy demonstrated by molecular imaging. PLoS One. 2012;7(9):e45776. doi: 10.1371/journal.pone.0045776. Epub 2012 Sep 20. [7]. Romero HK，et al. Inhibition of α9α10 nicotinic acetylcholine receptors prevents chemotherapy-induced neuropathic pain. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):E1825-E1832. |
| Unit | Bottle |
| Specification | 10mg 50mg |
Examples of using this product（for reference only）
In Vivo:
Mice ( 1.5 months old, male ICR mice; 5 mg/kg oxaliplatin; I.P；once a day for 10 d)：
According to the report, the CRC model mice were prepared by the established method, in which azoxymethane (AOM) and dextran sodium sulfate (DSS) were used. Mice were intraperitoneally injected with 2 mg/kg AOM once at the first week. Then the mice were given an aqueous solution of dissolved 2.5% DSS for one week. Two weeks later, mice received AOM injection again, followed by drinking DSS solution. The cycles were carried out for 5 rounds, with each round for 4 weeks. When the mice showed emaciation and hematochezia, they were randomly divided into four groups. The mice in one group were given PBS as model control, and the second group was intraperitoneally injected with 5 mg/kg oxaliplatin (positive control) once a day for 10 d. The other two groups were orally administered 5 mg/kg ph-ph+ and 5 mg/kg ph-ph+/SDC/EPC daily for 10 d.
Reference：
Zhang Y, Zhao Z, Li J, Wang Q, Fan Z, Yuan Z, Feng Y, Fu A. Treatment of colorectal cancer by anticancer and antibacterial effects of hemiprotonic phenanthroline-phenanthroline+ with nanomicelle delivery. Asian J Pharm Sci. 2023 May;18(3):100801. doi: 10.1016/j.ajps.2023.100801. Epub 2023 Mar 28. PMID: 37274926; PMCID: PMC10232661.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
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Zhang Y, et al. Asian J Pharm Sci. 2023 May;18(3):100801.
Zhang Y, et al. Asian J Pharm Sci. 2023 May;18(3):100801.
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Related Paper
Treatment of colorectal cancer by anticancer and antibacterial effects of hemiprotonic phenanthroline-phenanthroline+ with nanomicelle delivery
Click to check >>Author：Zhang Y, Zhao Z, Li J, Wang Q, Fan Z, Yuan Z, Feng Y, Fu A.
IF：10.2000
Publish_to：Asian J Pharm Sci
PMID：37274926
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