{{cart_num}}
My CartCAS：4373-41-5
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
Qty：
Size：
| CAS | 4373-41-5 |
| Name | Maslinic Acid |
| Molecular Formula | C30H48O4 |
| Molecular Weight | 472.7 |
| Solubility | Soluble in DMSO |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD00049293 |
| SMILES | C[C@]([C@@]1([H])[C@]2(C[C@@H](O)[C@@H]3O)C)(CC[C@@]2([H])C3(C)C)[C@@](C4=CC1)(CC[C@]5(C(O)=O)[C@@]4([H])CC(C)(C)CC5)C |
| InChIKey | MDZKJHQSJHYOHJ-LLICELPBSA-N |
| InChI | InChI=1S/C30H48O4/c1-25(2)12-14-30(24(33)34)15-13-28(6)18(19(30)16-25)8-9-22-27(5)17-20(31)23(32)26(3,4)21(27)10-11-29(22,28)7/h8,19-23,31-32H,9-17H2,1-7H3,(H,33,34)/t19-,20+,21-,22+,23-,27-,28+,29+,30-/m0/s1 |
| PubChem CID | 73659 |
| Target Point | p65 |
| Passage | NF-κB |
| Background | Maslinic acid inhibits the DNA-binding activity of NF-κB p65 and abrogates IκB-α phosphorylation. |
| Biological Activity | Maslinic acid 可抑制 NF-κB p65 的 DNA 结合活性, 并消除 IκB-α 磷酸化。Maslinic acid抑制LPS诱导的NF-κB易位至细胞核和IκB-α的磷酸化。[1]。 |
| In Vitro | 据报道，Maslinic acid可抑制骨髓单核细胞中NF-κB调节的破骨细胞生成，抑制TNF-α诱导的NF-κB活性及其下游基因在胰腺癌细胞中的表达。为了确认橄榄果渣提取物(OPE)在RAAW264.7细胞中的抗炎作用是否可归因于Maslinic acid，剂量依赖性实验确定Maslinic acid的有效浓度为10-20μM。 20μM的Maslinic acid显著抑制TNF-α的产生并抑制RAW 264.7细胞中的IL-1，IL-6和COX-2 mRNA表达。Maslinic acid(10和20μM)显著抑制LPS诱导的RAW 264.7细胞中NF-κBp65的DNA结合活性。用Maslinic酸预处理可显著降低LPS诱导的IκB-α磷酸化[1]。 |
| In Vivo | 与角叉菜胶诱导的对照组相比，在注射λ-角叉菜胶后4小时(分别为0.91±0.51 mm和1.79±0.4 mm)，给小鼠施用200 mg/kg Maslinic acid(MA)，显著抑制炎症，可减轻爪肿胀)[1]。 |
| Cell Experiment | 将RAW 264.7细胞以1×10 5个细胞/ mL的密度接种于96孔培养板中，培养24小时后，用OPE1(300μg/ mL或400μg/ mL)，OPE2(20μg/ mL)处理或40μg/ mL)，或Maslinic acid(10μM或20μM)，以及同时使用/不使用LPS。使用WST-1试剂测定细胞活力。简言之，将WST-1试剂(10μL)加入每个孔中并在潮湿的培养箱中孵育1小时。在450nm处测量样品的吸光度(参考波长为750nm)。活力表示为在LPS处理的细胞中测量的吸光度的百分比[1]。 |
| Animal Experiment | 小鼠[1]将5周龄雄性Balb/c小鼠(19-21g)以常规条件饲养，并提供对标准啮齿动物饲料和水的自由获取。通过足底内注射100μL1％角叉菜胶诱导水肿至左后爪。最初以200mg/kg的剂量测试Maslinic acid，在角叉菜胶注射之前和之后60分钟口服施用。在角叉菜胶处理后2，3和4小时使用电子数字卡尺测量爪厚度。在角叉菜胶注射后4小时，通过吸入二氧化碳处死小鼠[1]。 |
| Data Literature Source | [1]. Fukumitsu S，et al. Anti-inflammatory and anti-arthritic effects of pentacyclic triterpenoids maslinic acid through NF-κB inactivation. Mol Nutr Food Res. 2016 Feb;60(2):399-409. |
| Unit | Bottle |
| Specification | 5mg 10mM*1mL in DMSO 10mg |
Maslinic acid 可抑制 NF-κB p65 的 DNA 结合活性， 并消除 IκB-α 磷酸化。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
Manual Download
