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My CartCAS：4773-96-0
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Light yellow to yellow Solid
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| CAS | 4773-96-0 |
| Name | Mangiferin |
| Molecular Formula | C19H18O11 |
| Molecular Weight | 422.34 |
| Solubility | Soluble in DMSO |
| Purity | HPLC≥98% |
| Appearance | Light yellow to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 624-757-7 |
| MDL | MFCD00075656 |
| SMILES | O[C@@H]([C@H](O)[C@@H](CO)O1)[C@@H](O)[C@@H]1C2=C(O)C3=C(C=C2O)OC4=CC(O)=C(O)C=C4C3=O |
| InChIKey | AEDDIBAIWPIIBD-ZJKJAXBQSA-N |
| InChI | InChI=1S/C19H18O11/c20-4-11-15(25)17(27)18(28)19(30-11)12-8(23)3-10-13(16(12)26)14(24)5-1-6(21)7(22)2-9(5)29-10/h1-3,11,15,17-23,25-28H,4H2/t11-,15-,17+,18-,19+/m1/s1 |
| PubChem CID | 5281647 |
| Target Point | Nrf2;p65 |
| Passage | NF-κB |
| Background | Mangiferin is an Nrf2 activator. Mangiferin inhibits nuclear translocation of NF-κB subunits p65 and p50. |
| Biological Activity | Mangiferin 是一种 Nrf2 激活剂。Mangiferin 抑制 NF-κB 亚基 p65 和 p50 的核移位。[1-2] |
| In Vitro | 芒果苷是从漆树科(Anacardiaceae)和龙胆科(Gentianaceae)植物中提取的葡糖基黄酮酮。芒果甙(50μM)显著增加HL-60细胞中Nrf2蛋白的积累，特别是在细胞核中。芒果甙还增强Nrf2与抗氧化反应元件(ARE)的结合，显著上调NAD(P)H：醌氧化还原酶1(NQO1)表达并降低HL60细胞中的细胞内ROS。芒果甙单独剂量依赖性地抑制HL-60细胞的增殖。在单核细胞(MNCs)中，芒果甙显著减轻氧化应激，但减弱依托泊苷诱导的细胞毒性[1]。芒果甙是一种天然的植物多酚，具有多种药理特性。芒果甙存在于几种植物物种中，例如芒果(Mangifera indica)，鸢尾(Iris unguicularis)和知母(Anemarrhena asphodeloides)。芒果甙通过抑制NF-κB活性下调TNF-α诱导的MMP-9 mRNA和蛋白表达，从而抑制LNCaP细胞的侵袭。为了评估芒果甙是否影响LNCaP细胞的存活，MTT测定在不同处理后24小时进行。在血清和无血清条件下存在或不存在TNF-α时芒果苷的浓度。在血清和无血清条件下，单独用高达400μM的芒果甙处理的LNCaP细胞中没有明显的细胞毒性。另外，在存在TNF-α(20ng/mL)的情况下，芒果甙不影响细胞活力。芒果甙(400μM)处理显著降低TNF-α刺激的LNCaP细胞中的NF-κB荧光素酶活性。用芒果甙(400μM)预处理1小时显著降低TNF-α诱导的NF-κB活性。 RT-PCR显示芒果甙(400μM)显著抑制LNCaP细胞中TNF-α诱导的MMP-9表达[2]。 |
| Cell Experiment | LNCaP细胞在37℃，5％CO 2下在补充有10％FBS和抗生素的RPMI中培养。通过MTT测定确定细胞活力。简而言之，将LNCaP细胞(1×10 5个细胞/ mL)接种到24孔板上，并在血清和无血清RPMI培养基中孵育过夜。用指定浓度的芒果甙(100，200和400μM)处理细胞1小时，然后用TNF-α(20ng/mL)刺激24小时。然后，将细胞与0.5mg/mL MTT的溶液一起温育，并在37℃和5％CO 2下孵育45分钟。除去上清液，通过使用酶标仪在540nm处监测信号来观察甲forma的形成[2]。 |
| Data Literature Source | [1]. Zhang BP，et al. Mangiferin activates Nrf2-antioxidant response element signaling without reducing the sensitivity to etoposide of human myeloid leukemia cells in vitro. Acta Pharmacol Sin. 2014 Feb;35(2):257-66. [2]. Dilshara MG，et al. Mangiferin inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 expression and cellular invasion by suppressing nuclear factor-κB activity. BMB Rep. 2015 Oct;48(10):559-64 |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg |
Mangiferin 是一种 Nrf2 激活剂。Mangiferin 抑制 NF-κB 亚基 p65 和 p50 的核移位。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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