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My CartCAS：28822-58-4
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥99%
Appearance：White to off-white Solid
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| CAS | 28822-58-4 |
| Name | IBMX |
| Molecular Formula | C10H14N4O2 |
| Molecular Weight | 222.24 |
| Solubility | Soluble in DMSO ≥10mg/mL;Soluble in Ethanol ≥10mg/mL |
| Purity | HPLC≥99% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 249-259-3 |
| MDL | MFCD00005584 |
| SMILES | O=C(N1C)N(CC(C)C)C2=C(N=CN2)C1=O |
| InChIKey | APIXJSLKIYYUKG-UHFFFAOYSA-N |
| InChI | InChI=1S/C10H14N4O2/c1-6(2)4-14-8-7(11-5-12-8)9(15)13(3)10(14)16/h5-6H,4H2,1-3H3,(H,11,12) |
| PubChem CID | 3758 |
| Target Point | Phosphodiesterase (PDE) |
| Passage | Metabolic Enzyme&Protease |
| Background | IBMX is a broad-spectrum, nonspecific phosphodiesterase (PDE) inhibitor. Can enhance intracellular cAMP levels, is an Adenosine (A1) receptor antagonist. Can inhibit the reduction of tryptamine induced by phenylephrine. |
| Biological Activity | IBMX 是一种广谱的磷酸二酯酶 (PDE) 抑制剂, 抑制 PDE3, PDE4 和 PDE5, IC50 分别为 6.5, 26.3 和 31.7 μM。[1] |
| IC50 | IC50: 6.5±1.2μM(PDE3) ， 26.3±3.9μM (PDE4) ， 31.7±5.3μM (PDE5) [1] |
| In Vitro | 在100μM时，KMUP-1(黄嘌呤衍生物)和IBMX在诱导气管松弛方面最有效; KMUP-1和IBMX引起的松弛反应的幅度没有显着差异[1]。 IBMX(100μM)激活肾外髓质K +(ROMK)通道(n = 6，P <0.05)并阻止ANG II(n = 6，P = NS)或cGMP进一步激活通道。值得注意的是，使用IBMX(100μM)从高K +(HK)喂养的大鼠中分离皮质集合管(CCD)20分钟导致管状cAMP含量显着增加至1.43±0.35 pg / mm小管长度(n = 14)与载体处理的对照组相比(0.61±0.13 pg / mm小管长度，n = 12，P <0.05)[2]。 |
| In Vivo | IBMX是一种非选择性PDE抑制剂，可显着降低肝糖原储存量(mg / g，IBMX 22±1.5 P 0.05)mc2也不会改变血浆葡萄糖(对照= 141±3，mc2 = 145±5)。 IBMX在增加血浆葡萄糖方面效果最好[3]。使用IBMX和Apocynin治疗可显着降低冷诱导的右心室(RV)收缩压升高(分别为23.5±1.8和24.2±0.6 mmHg)，尽管它们不会将RV压力降低至温控水平。 IBMX或Apocynin显着降低了中层厚度(分别为19.0±0.9和16.9±0.8μm)，并且增加了冷暴露大鼠中小PA的管腔直径(分别为62.7±4.2和59.5±4.3μm)[4]。 |
| Cell Experiment | 细胞在24孔板中生长，每孔105个细胞。在汇合时，用磷酸盐缓冲溶液(PBS)洗涤单层细胞，然后在100μMIBMX存在下与KMUP-1(0.1-100μM)一起温育20分钟。通过加入10％三氯乙酸(TCA)终止孵育。将细胞悬浮液超声处理，然后在4℃下以2500×g离心15分钟。为了除去TCA，将上清液用5倍体积的水饱和的乙醚萃取三次。然后，将上清液冻干，并使用市售的放射免疫测定试剂盒[2]测定每个样品的环状GMP或AMP。 |
| Animal Experiment | 小鼠[3]使用雄性小鼠(25-35g)。对于实验，将测试化合物(IBMX，米力农，MCPIP，mc1，mc2，mc5或mc6)或溶剂(对照)以1mg / kg剂量皮下注射给小鼠，每天两次(上午8:00和8:00)下午7点。在第8天，通过腹膜内注射硫喷妥钠(80mg / kg)麻醉动物，从心脏获得血液样品，然后解剖肝脏。将每个样品离心5分钟并分离其血清。每只动物的血清和肝脏在低于-18℃的温度下保持冷冻用于以下测量。大鼠[4]使用六组雄性Sprague-Dawley大鼠(150-180g，6只大鼠/组)。将三组大鼠暴露于保持中度冷(5.0±1℃)的气候控制的步入室中。将剩余的组保持在保持室温(23.5±1℃，温热)的相同室中并用作对照。在暴露于寒冷8周后，在每个温度条件下3组接受连续IV输注IBMX(PDE-1抑制剂，8.5mg / kg /天)，Apocynin(NADPH氧化酶抑制剂，25mg / kg /天)和载体(DMSO，50％)。已经证实药物剂量分别有效抑制PDE-1和NADPH氧化酶活性。每周测量体重。药物输注一周后，在麻醉下测量动物的右心室收缩压(RVBP)。 RVP是肺动脉血压(PAP)的可靠指标，并且已被许多研究者用于评估PH。 |
| Data Literature Source | [1]. Wu BN， et al. KMUP-1， a xanthine derivative， induces relaxation of guinea-pig isolated trachea: the role of the epithelium， cyclic nucleotides and K+ channels. Br J Pharmacol. 2004 Aug; 142 (7) :1105-14 [2]. Wei Y， et al. Angiotensin II type 2 receptor regulates ROMK-like K+ channel activity in the renal cortical collecting duct during high dietary K+ adaptation. Am J Physiol Renal Physiol. 2014 Oct 1; 307 (7) :F833-43 [3]. Hosseini A， et al. Differential metabolic effects of novel cilostamide analogs， methyl carbostiryl derivatives， on mouse and hyperglycemic rat. Iran J Basic Med Sci. 2012 Jul; 15 (4) :916-25. [4]. Crosswhite P， et al. Inhibition of phosphodiesterase-1 attenuates cold-induced pulmonary hypertension. Hypertension. 2013 Mar; 61 (3) :585-92 |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg 100mg |
Examples of using this product（for reference only）
In Vitro:
Cell（ 0.5 mM isobutyl-methylxanthine）:
The adipogenic medium was the complete culture medium supplemented with 1 μM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/L insulin (Solarbio), and 0.5 mM isobutyl-methylxanthine (Solarbio).
Reference：
Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.
Mycelia（2.5 mM IBMX）:
To test the effect of cAMP and IBMX on appressorium formation of the Δpal1 deletion mutant, spore suspension (105 spores/ml) was inoculated on a hydrophobic glass slide and then 1 mM cAMP and 2.5 mM IBMX (Solarbio) were added to observe the appressorium formation ratio at different time points.
Reference：
Chen D, Hu H, He W, Zhang S, Tang M, Xiang S, Liu C, Cai X, Hendy A, Kamran M, Liu H, Zheng L, Huang J, Chen XL, Xing J. Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae. Mol Plant Pathol. 2022 Jan;23(1):133-147. doi: 10.1111/mpp.13149. Epub 2021 Oct 12. PMID: 34636149; PMCID: PMC8659611.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Chen D, et al. Mol Plant Pathol. 2022 Jan;23(1):133-147.
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