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My CartCAS：53963-43-2
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 53963-43-2 |
| Name | Ginsenoside F1 |
| Molecular Formula | C36H62O9 |
| Molecular Weight | 638.88 |
| Solubility | Soluble in DMSO |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| MDL | MFCD06410947 |
| SMILES | C[C@]([C@@](C[C@H]1O)([H])[C@]2(CC[C@@H]3O)C)(C[C@H](O)[C@@]2([H])C3(C)C)[C@]4([C@@]1([H])[C@]([C@@](CC/C=C(C)/C)(C)O[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO)([H])CC4)C |
| InChIKey | XNGXWSFSJIQMNC-FIYORUNESA-N |
| InChI | InChI=1S/C36H62O9/c1-19(2)10-9-13-36(8,45-31-29(43)28(42)27(41)23(18-37)44-31)20-11-15-34(6)26(20)21(38)16-24-33(5)14-12-25(40)32(3,4)30(33)22(39)17-35(24,34)7/h10,20-31,37-43H,9,11-18H2,1-8H3/t20-,21+,22-,23+,24+,25-,26-,27+,28-,29+,30-,31-,33+,34+,35+,36-/m0/s1 |
| PubChem CID | 9809542 |
| Target Point | Cytochrome P450 |
| Passage | Metabolic Enzyme&Protease |
| Background | Ginsenoside F1 competitively inhibits CYP3A4. |
| Biological Activity | Ginsenoside F1 是 Ginsenoside Rg1 的酶促修饰衍生物，Ginsenoside F1 竞争性抑制 CYP3A4，对 CYP2D6 具有较弱的抑制作用。[1-2] |
| In Vitro | 已经显示人参皂甙F1具有抗癌，抗衰老和抗氧化作用，并且已经证明了CYP3A4活性的竞争性抑制和对CYP2D6活性的较弱抑制。在MTT试验中，最高浓度的人参皂苷F1(200μM)的细胞活力为68％[1]。 |
| In Vivo | 给ApoE - / - 小鼠喂食高脂肪饮食，并用人参皂苷F1(50mg / kg /天)口服治疗8周。与模型组小鼠相比，人参皂甙F1处理的小鼠显着减少了病变大小[2]。 |
| Cell Experiment | 将B16BL6细胞在补充有10％胎牛血清和1％青霉素 - 链霉素的Dulbecco改良的Eagles培养基中于37℃在湿润的95％空气/ 5％CO 2气氛中培养。使用MTT转化成甲，测定人参皂苷F1和代谢物1的细胞活力。将细胞以1×10 5个细胞/孔的密度接种在96孔板中，培养24小时，并用各种浓度的1μM至200μM的人参皂苷F1和代谢物1处理5天。最后，向每个孔中加入10μLMTT(PBS中5mg / mL)。将细胞在37℃下孵育3小时，然后加入DMSO(100μL)以溶解甲crystals晶体。使用ELISA读数器在570nm处测量吸光度，参比波长为630nm [1]。 |
| Animal Experiment | 小鼠[2]将6周龄(17±1 g)雄性C57BL / 6小鼠和具有C57BL / 6背景的ApoE - / - 小鼠维持在温度控制的设施中(温度：22±1°C; 湿度)：60％)在常规笼中具有14小时光照/ 10小时黑暗循环。将40只小鼠随机分成4个实验组(n = 10 /组):(I)C57BL / 6N小鼠，对照组;(II)ApoE - / - 小鼠组;(III)ApoE - / - 小鼠+人参皂苷F1组;(IV)ApoE - / - 小鼠+普罗布考组。给所有小鼠喂食高脂肪饮食(HFD，0.3％胆固醇和20％猪肉脂肪)8周。将人参皂甙F1(50mg / kg /天，ig)和普罗布考(2g / kg，ig)溶解在羧甲基纤维素钠(CMC-Na)中。每天给小鼠口服给药8周。对照组和模型组每天接受无菌0.5％CMC-Na处理(ig，0.1 mL / 10g)[2]。 |
| Kinase Experiment | 用过表达的BSGT1酶和F1测定糖基化能力。反应混合物含有100μL的0.5mM F1和100μL的2.5mM UDP-葡萄糖和800μL纯化的酶(终浓度为0.1mg / mL)(pH7.0)。将混合物在30℃下孵育24小时。此外，在相同条件下孵育三组对照：(1)对照1(C1)由人参皂苷F1和BSGT1组成;(2)对照2(C2)由具有UDP-葡萄糖的BSGT1组成;(3)对照3(C3)由人参皂甙F1和UDP-葡萄糖组成[1]。 |
| Data Literature Source | [1]. Wang DD， et al. Rare ginsenoside Ia synthesized from F1 by cloning and overexpression of the UDP-glycosyltransferase gene from Bacillus subtilis: synthesis， characterization， and in vitromelanogenesis inhibition activity in BL6B16 cells. J Ginseng Res. 2018 Jan; 42 (1) :42-49. [2]. Qin M， et al. Ginsenoside F1 Ameliorates Endothelial Cell Inflammatory Injury and Prevents Atherosclerosis in Mice through A20-Mediated Suppression of NF-kB Signaling. Front Pharmacol. 2017 Dec 22; 8:953 |
| Unit | Bottle |
| Specification | 5mg 10mM*1mL in DMSO 10mg |
Ginsenoside F1 竞争性抑制 CYP3A4。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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