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My Cart> Small Molecule Compounds > Reaction Substrate & Physicochemical Detection > X-GALCAS：7240-90-6
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to off-white Solid
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| CAS | 7240-90-6 |
| Name | X-GAL |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.63 |
| Solubility | Soluble in DMF/DMSO |
| Purity | ≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 230-640-8 |
| MDL | MFCD00005666 |
| SMILES | O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=CNC3=C2C(Cl)=C(Br)C=C3 |
| InChIKey | OPIFSICVWOWJMJ-AEOCFKNESA-N |
| InChI | InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1 |
| PubChem CID | 65181 |
| Background | X-GAL is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until it is cleaved by beta-galactomidase， after which X-GAL becomes an insoluble and detectable blue compound， which makes X-GAL useful in techniques such as blue-white screening for bacterial cloning. X-GAL can also be used to detect beta-galactosidase activity. |
| Unit | Bottle |
| Specification | 50mg 100mg |
X-gal is the substrate of β-Galactosidase, which is blue when hydrolyzed. Based on this characteristic, when pUC series carrier DNA (or other carrier DNA with lacZ gene) is transformed by LACz-deficient cells as host, or transfected with M13 phage carrier DNA, if X-Gal and IPTG are added to plate culture medium, due to the α-complementality of β-galactosidase, Recombinants can be easily selected based on the presence or absence of white colonies (or plaque).
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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