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My CartCAS：3326-32-7
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥97%
Appearance：Light yellow to orange yellow Solid
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| CAS | 3326-32-7 |
| Name | FITC |
| Molecular Formula | C21H11NO5S |
| Molecular Weight | 389.38 |
| Solubility | Soluble in DMSO ≥10mg/mL;Soluble in Ethanol ≥1mg/mL;Soluble in Water ＜1mg/mL |
| Purity | HPLC≥97% |
| Appearance | Light yellow to orange yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 248-207-7 |
| MDL | MFCD00005063 |
| SMILES | O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N=C=S)C=C5 |
| InChIKey | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| InChI | InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| PubChem CID | 18730 |
| Background | FITC?is?a?derivative?of?fluorescein?used?to?label?amines.?Ex=488nm，Em=525nm. |
| Biological Activity | FITC是荧光素的衍生物，用于标记胺[1-2]。 |
| In Vitro | Annexin V-FITC/PI用于检测软骨细胞凋亡[1]。 PEI修饰的CD与异硫氰酸荧光素(FITC)缀合以制备CDs-FITC复合物[2]。 |
| Cell Experiment | 用膜联蛋白V-荧光素异硫氰酸酯(FITC)和碘化丙啶通过流式细胞术测量软骨细胞凋亡的频率。从每组中收集总共1×104个处理的软骨细胞，在冷PBS中洗涤并在室温下与膜联蛋白V-FITC和PI在黑暗中在冰上孵育15分钟。然后使用荧光激活细胞分选仪分析这些样品。 Cell Quest软件用于分析细胞凋亡的百分比。所有测试重复三次。 |
| Data Literature Source | [1]. Gao SG，et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5. [2]. Zhu X，et al. Ratiometric，visual，dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71 |
| Unit | Bottle |
| Specification | 100mg 10mM*1mL in DMSO 500mg |
Examples of using this product（for reference only）
In Vitro:
Phagocytosis Assay of Hemocyte（FITC-labeled C. albicans (1×105 cells, 5 μl)，37℃ for 3 h in dark ）:
In brief, 15 larvae per group were inoculated with 5 μl of PBS or ZnO-NP suspensions (2 mg/kg) via the last left proleg. After incubation at 37℃ for 2 h, each larva was challenged with fluorescein isothiocyanate isomer I (FITC; Solarbio, Beijing, China)-labeled C. albicans (1×105 cells, 5 μl) and incubated at 37℃ for 3 h in dark. For preparing FITC-labeled C. albicans, C. albicans suspensions (1×109 cells/ml) were incubated with 0.1 mg/ml in a shaking incubator in the dark for 1 h and washed with PBS three times. Subsequently, 50 μl of hemolymph was obtained from each larva and diluted in a 12-well plate containing 950 μl of IPS and 0.002% PTU. The plate was gently shaken followed by standing for 15 min at room temperature. The phagocytosis of C. albicans by hemocytes was visualized using an IX73 fluorescence microscope.
Reference：
Xu MN, Li L, Pan W, Zheng HX, Wang ML, Peng XM, Dai SQ, Tang YM, Zeng K, Huang XW. Zinc Oxide Nanoparticles Prime a Protective Immune Response in Galleria mellonella to Defend Against Candida albicans. Front Microbiol. 2021 Dec 10;12:766138. doi: 10.3389/fmicb.2021.766138. PMID: 34956129; PMCID: PMC8702860.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Wang M , Bi S , Pang J ,et al.Precise quantification of the antibacterial activity of chitosan by NB medium neutralizer,2021.
Xu MN, et al. Front Microbiol. 2021 Dec 10;12766138.
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