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My CartCAS：76823-03-5
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥95%
Appearance：Light yellow to yellow Solid
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| CAS | 76823-03-5 |
| Name | 5-FAM |
| Molecular Formula | C21H12O7 |
| Molecular Weight | 376.32 |
| Solubility | Soluble in DMSO |
| Purity | HPLC≥95% |
| Appearance | Light yellow to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 276-331-1 |
| MDL | MFCD00036874 |
| SMILES | O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(C(O)=O)C=C5 |
| InChIKey | NJYVEMPWNAYQQN-UHFFFAOYSA-N |
| InChI | InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25) |
| PubChem CID | 123755 |
| Background | 5-FAM is an isomer of carboxyfluorescein. |
| In Vitro | 5-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity，excellent fluorescence quantum yield，and good water solubility，fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser[1]. |
| Data Literature Source | [1]. Jungmi Lee，et al. Peptide substrate-based inkjet printing high-throughput MMP-9 anticancer assay using fluorescence resonance energy transfer (FRET). Sens Actuators B Chem. 2018 Mar;256:1093-1099. |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg |
5-FAM是羧基荧光素的一个同分异构体。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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