Estradiol

Examples of using this product（for reference only）

In Vitro:

Cell（epidermal cells and skin-derived precursors (SKPs)，20 nM b-estradiol）：

Formation of HFS and IHFSAfter collected in isolation procedure, the epidermal cells and SKPs were mixed at ratio of 1:5e9. Then the mixture was seeded into 20 ml HF induction culture medium (DMEM/F12;10% (v/v) FBS, 1% (v/v) Insulin-transferrin-selenium,0.2 nM 3,30,5-Triiodo-L-thyronine, 20 ng/ml basic fibroblast growth factor, 2 nM hydrocortisone hemisuccinate, 2 nM testosterone, Solarbio, China; 20 nM b-estradiol, Solarbio, China; 20 nM progesterone, Solarbio, China) droplet on the lid of 100 mm dish with a total cell of 5×10⁵ in each droplet. When the lid was put upside down, the cells sedimented at the bottom of the droplet and aggregated to form HFS after 24 h culture. The culture medium was changed every other day. After 7 days culture, the HFS was collected.

Reference：

Zhang Y, Enhejirigala, Li Z, Song W, Yao B, Li J, Wang Y, Duan X, Yuan X, Huang S, Fu X. The role of CTHRC1 in hair follicle regenerative capacity restored by plantar dermis homogenate. Biochem Biophys Res Commun. 2021 Sep 24;571:14-19. doi: 10.1016/j.bbrc.2021.07.056. Epub 2021 Jul 20. PMID: 34298337.

Cell（hAMSCs；1×10^-6 mol/L β-estradiol；3d）：

For endometrial diferentiation, when hAMSCs reached 30–40% confuence, the medium was exchanged for DMEM/F12 supplemented with 2%FBS, 10 ng/mL TGF-β1, 10 ng/mL EGF, 10 ng/mL PDGF-BB, 1× 10^-6 mol/L β-estradiol (Solarbio), and endometrial conditioned medium. Uninduced cells were cultured with DMEM/F12 containing 2% FBS. After 3 days, the expression of CK7 and CK19 was detected by immunofuorescence.

Reference：

Huang J, Zhang W, Yu J, Gou Y, Liu N, Wang T, Sun C, Wu B, Li C, Chen X, Mao Y, Zhang Y, Wang J. Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration. Stem Cell Res Ther. 2022 Jan 12;13(1):17. doi: 10.1186/s13287-021-02682-2. PMID: 35022063

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.