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My CartCAS：114-07-8
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 114-07-8 |
| Name | Erythromycin |
| Molecular Formula | C37H67NO13 |
| Molecular Weight | 733.93 |
| Solubility | Soluble in DMSO/Ethanol |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 204-040-1 |
| MDL | MFCD00084654 |
| SMILES | C[C@@H]([C@@H]([C@H](C(O[C@@H]1CC)=O)C)O[C@@](O[C@@H](C)[C@@H]2O)([H])C[C@@]2(C)OC)[C@H]([C@](O)(C[C@H](C([C@@H]([C@@H](O)[C@@]1(O)C)C)=O)C)C)O[C@@](O[C@H](C)C[C@@H]3N(C)C)([H])[C@@H]3O |
| InChIKey | ULGZDMOVFRHVEP-RWJQBGPGSA-N |
| InChI | InChI=1S/C37H67NO13/c1-14-25-37(10,45)30(41)20(4)27(39)18(2)16-35(8,44)32(51-34-28(40)24(38(11)12)15-19(3)47-34)21(5)29(22(6)33(43)49-25)50-26-17-36(9,46-13)31(42)23(7)48-26/h18-26,28-32,34,40-42,44-45H,14-17H2,1-13H3/t18-,19-,20+,21+,22-,23+,24+,25-,26+,28-,29+,30-,31+,32-,34+,35-,36-,37-/m1/s1 |
| PubChem CID | 12560 |
| Target Point | Bacterial |
| Passage | Anti-infection |
| Background | It is a macrolide antibiotic that can reversibly bind to bacterial 50S ribosomes and inhibit protein synthesis. |
| Unit | Bottle |
| Specification | 10mM*1mL in DMSO 500mg 1g |
Examples of using this product（for reference only）
In Vitro：
E. coli（3 μg/mL erythromycin）:
After purification from E. coli positive strains, the resulting plasmids, pMAD-?cheAY, were electroporated into L. monocytogenes competent cells and also in the in-frame ?cheAY mutant with the selection performed on TSA-containing erythromycin (3 μg/mL). Using erythromycin as the screening condition, the L. monocytogenes strain containing pMAD-?cheAY plasmid was subcultured continuously to realize homologous recombination.
Reference：
Li Q, Guo A, Ma Y, Liu L, Liu W, Zhong Y, Zhang Y. Gene Analysis of Listeria monocytogenes Suspended Aggregates Induced by Ralstonia insidiosa Cell-Free Supernatants under Nutrient-Poor Environments. Microorganisms. 2021 Dec 15;9(12):2591. doi: 10.3390/microorganisms9122591. PMID: 34946191; PMCID: PMC8704912.
E. coli（100/200 μg/mL erythromycin）:
The culture of L. plantarum SL09 strain with plasmid pMG36e or pMG36eargG required the addition of erythromycin (Solarbio, Beijing, China), with a final concentration of 100 μg/ml, meanwhile, the E. coli DH5α strain with plasmid pMG36e or pMG36eargG required the addition of erythromycin (Solarbio, Beijing, China) with a final concentration to 200 μg/ml.
Reference：
Zhao H, Liu L, Peng S, Yuan L, Li H, Wang H. Heterologous Expression of Argininosuccinate Synthase From Oenococcus oeni Enhances the Acid Resistance of Lactobacillus plantarum. Front Microbiol. 2019 Jun 21;10:1393. doi: 10.3389/fmicb.2019.01393. PMID: 31293541; PMCID: PMC6598401.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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