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My CartCAS：518-82-1
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Yellow to orange Solid
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| CAS | 518-82-1 |
| Name | Emodin |
| Molecular Formula | C15H10O5 |
| Molecular Weight | 270.24 |
| Solubility | Soluble in DMSO ≥5mg/mL |
| Purity | HPLC≥98% |
| Appearance | Yellow to orange Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 208-258-8 |
| MDL | MFCD00001207 |
| SMILES | O=C1C2=C(C=C(C)C=C2O)C(C3=CC(O)=CC(O)=C31)=O |
| InChIKey | RHMXXJGYXNZAPX-UHFFFAOYSA-N |
| InChI | InChI=1S/C15H10O5/c1-6-2-8-12(10(17)3-6)15(20)13-9(14(8)19)4-7(16)5-11(13)18/h2-5,16-18H,1H3 |
| PubChem CID | 3220 |
| Target Point | Casein Kinase（CK） |
| Passage | Cell Cycle;DNA Damage/DNA Repair; Stem Cells |
| Background | Emodin is a protein kinase CK2 inhibitor and an effective selective11 β- HSD1 inhibitor has a broad spectrum of anticancer activity. |
| Biological Activity | Emodin 是一种广谱抗癌剂。Emodin是一种Ser/Thr激酶，选择性地抑制酪蛋白激酶II（CKII）。大黄素抑制CKII活性，IC50为2μM。[1-3] |
| In Vitro | 酶动力学测定表明，大黄素抑制CKII活性作为对ATP的竞争性抑制剂，Ki为7.2μM[1]。大黄素是癌细胞的广谱抑制剂，包括白血病，肺癌，人舌鳞癌，结肠癌，胆囊癌，胰腺癌，乳腺癌，人宫颈癌和肝癌细胞。大黄素抑制A549，HepG2，OVCAR-3，HeLa和Madin-Darby犬肾(MDCK)细胞，IC50分别为19.54，12.79，25.82，12.14和5.81μg/ mL。大黄素的抗癌机制涉及许多生物学途径，如酪蛋白激酶II和ERK1/2 [2]。大黄素作为活性氧(ROS)发生器与T24和J82人膀胱癌细胞中的顺铂组合应用。大黄素以剂量依赖性和时间依赖性方式杀死T24和J82细胞，对HCV-29细胞的毒性较小。选择20和15μM的浓度作为适当剂量，分别在24小时研究T24和J82细胞的化学治疗敏感性[3]。 |
| In Vivo | 用大黄素(50mg/kg)和顺铂(1mg/kg)治疗的小鼠具有比来自其他组的小鼠显著更小的肿瘤。此外，各组之间未观察到体重减轻的显著差异，并且在肝，肾和心脏切片中未观察到明显的坏死和异常。这些结果表明，大黄素/顺铂共处理可显著抑制体内肿瘤生长，没有明显的副作用。与体外实验一致，TUNEL测定显示大黄素/顺铂组合显著增加异种移植肿瘤中的细胞凋亡。大黄素/顺铂联合治疗组的MRP1表达也低于其他组[3]。 |
| Cell Experiment | 将T24人膀胱癌细胞，HCV-29正常膀胱上皮细胞和J82人膀胱癌细胞在补充有10％胎牛血清的RPMI 1640培养基中于37℃在含有5％CO 2的潮湿气氛中培养。将细胞接种在96孔板中，每孔2×10 4个细胞。将细胞与大黄素以不同浓度(0，5，10，20，30，40，50，60，70μM)孵育24小时，并选择用细胞处理的临界浓度(20μM)0，6，12，24，48，72，96小时。将细胞与顺铂一起在不同浓度(0，0.5，1.0，1.5，2.0，2.5，3.0μg/ mL)下孵育24小时。 MTT测定用于分析细胞活力。用药物处理细胞24小时，并使用AnnexinV-异硫氰酸荧光素(AnnexinV-FITC)/碘化丙啶(PI)试剂盒用流式细胞术评估细胞凋亡率。根据制造商的说明制备样品并通过流式细胞术(FCM)Calibur分析[3]。 |
| Animal Experiment | 收获小鼠[3] 3×106T24细胞，洗涤并重悬于无血清最佳培养基中，然后皮下注射到6周龄BALB/c-nu/nu小鼠(每组n = 8只小鼠)中。接种后3天，每两天给小鼠腹膜内施用PBS，大黄素(50mg/kg)，顺铂(1mg/kg)或大黄素/顺铂。在第18天，每只小鼠都被处死。测量体重后，分离肿瘤，称重并固定在4％多聚甲醛(PFA)中。用苏木精和曙红染色心脏，肝脏和肾脏以确定全身毒性。末端脱氧核苷酸转移酶(TdT)介导的dUTP缺口末端标记(TUNEL)测定在多聚甲醛固定和石蜡包埋的肿瘤切片上进行。 |
| Data Literature Source | [1]. Yim H，et al. Emodin，an anthraquinone derivative isolated from the rhizomes of Rheum palmatum，selectively inhibits the activity of casein kinase II as a competitive inhibitor. Planta Med. 1999 Feb;65(1):9-13. [2]. Xing JY，et al. Antitumor Effects and Mechanism of Novel Emodin Rhamnoside Derivatives against Human Cancer CellsIn Vitro. PLoS One. 2015 Dec 18;10(12):e0144781. [3]. Li X，et al. Emodin enhances cisplatin-induced cytotoxicity in human bladder cancer cells through ROS elevation and MRP1 downregulation. BMC Cancer. 2016 Aug 2;16:578 |
| Unit | Bottle |
| Specification | 20mg 10mM*1mL in DMSO 50mg |
Examples of using this product（for reference only）
In Vitro:
Cell (HPDE6?C7 cells; 5.625~180 μM emodin; 24 h ):
The HPDE6?C7 cells (1x105 cells/mL) were treated with gradient concentrations of emodin (180, 90, 45, 22.5, 11.25 and 5.625 μM)(Solarbio) for 24 h in a humidified atmosphere of 5% CO2 at 37?C. Emodin was dissolved in DMSO to a final concentration of<0.1%. Finally, the MTT method was used to evaluate cell viability as described above.
Reference：
Zhang Q, Hu F, Guo F, Zhou Q, Xiang H, Shang D. Emodin attenuates adenosine triphosphate?induced pancreatic ductal cell injury In Vitro
via the inhibition of the P2X7/NLRP3 signaling pathway. Oncol Rep. 2019 Oct;42(4):1589-1597. doi: 10.3892/or.2019.7270. Epub 2019 Aug 8. PMID: 31524270.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Zhang Q, et al. Oncol Rep. 2019 Oct;42(4):1589-1597.
Zhang Q, et al. Oncol Rep. 2019 Oct;42(4):1589-1597.
Zhou Q, et al. Pancreas. 2021 Sep 1;50(8):1202-1211.
Zhou Q, et al. Pancreas. 2021 Sep 1;50(8):1202-1211.
Zhou Q, et al. Pancreas. 2021 Sep 1;50(8):1202-1211.
Zhou Q, et al. Pancreas. 2021 Sep 1;50(8):1202-1211.
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