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My Cart> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Endocrinology & Hormones > DHEACAS：53-43-0
Molecular Formula：C19H28O2
Molecular Weight：288.42
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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Size：
| CAS | 53-43-0 |
| Name | DHEA |
| Molecular Formula | C19H28O2 |
| Molecular Weight | 288.42 |
| Solubility | Soluble in DMSO ≥5mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 200-175-5 |
| MDL | MFCD00003613 |
| SMILES | O=C1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C |
| InChIKey | FMGSKLZLMKYGDP-USOAJAOKSA-N |
| InChI | InChI=1S/C19H28O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h3,13-16,20H,4-11H2,1-2H3/t13-,14-,15-,16-,18-,19-/m0/s1 |
| PubChem CID | 5881 |
| Target Point | Androgen Receptor |
| Passage | Endocrinology & Hormones |
| Background | DHEA is an important endogenous steroid hormone that can be used as an androgen receptor antagonist and an estrogen receptor agonist. |
| Biological Activity | DHEA是最丰富的类固醇激素之一。 DHEA通过多种信号传导途径介导其作用，并通过转化成雄激素和雌激素衍生物（例如，雄激素，雌激素，7α和7β DHEA，以及7α和7β表雄甾酮衍生物）通过其特异性受体起作用[1-3]。 |
| In Vitro | DHEA是一种有效的抗细胞凋亡因子，可逆转前列腺癌细胞(DU145和LNCaP细胞系)以及结肠癌细胞(Caco2细胞系)中血清剥夺诱导的细胞凋亡。 DHEA显著降低所有3种癌细胞类型中血清剥夺诱导的细胞凋亡，用APOPercentage测定法定量(分别用DHEA或NGF处理12小时后细胞凋亡从0.587±0.053降至0.142±0.0016或0.059±0.002; n = 3，P <0.01)，并通过流式细胞术分析(FACS)检测DU145细胞。 DHEA的抗细胞凋亡作用是剂量依赖性的，纳摩尔浓度下的EC50(分别在DU145和Caco2细胞中EC50：11.2±3.6nM和12.4±2.2nM)[1]。 DHEA是人类的主要性类固醇前体，可以直接转化为雄激素。如通过胸苷掺入测定所评估的，DHEA(≥1μM)引起Chub-S7增殖的剂量依赖性抑制。 DHEA处理以剂量依赖性方式抑制关键的糖皮质激素调节基因H6PDH(≥100nM)和HSD11B1(≥1μM)在分化前脂肪细胞中的表达。与此结果一致，DHEA处理(≥1μM)导致11β-HSD1氧化还原酶活性(≥1μM)显著降低，并且在分化的脂肪细胞中使用的最高DHEA剂量(25μMDHEA)下脱氢酶活性同时增加[2]。 |
| In Vivo | 与喂食对照AIN-76A饮食的小鼠相比，处理8周的雄性B6小鼠(5只小鼠组)的饮食中的DHEA(0.45％w/w)导致体温显著降低。类似的比较表明对照和配对喂养的小鼠也有显著差异。喂食DHEA的动物的温度显著低于喂食对照饮食的小鼠26/29次测试;饲喂DHEA饮食的小鼠对受影响较小，8/29值显著低于随意喂食AIN-76A的小鼠。喂食DHEA或喂食DHEA的小鼠的温度在测试中显著不同21/29倍。喂食对照饮食的小鼠的体重显著高于喂食DHEA或喂给DHEA的小鼠的体重。除第9周(n = 3)外，每周平均摄取来自笼子的食物摄入量(克/天)(n = 7)。喂食DHEA的小鼠的食物摄入量显著减少。通过设计，喂给DHEA的小鼠配对吃了大约相同的量。因此，似乎DHEA通过食物限制和单独的机制降低体温[3]。 |
| Cell Experiment | 将Chub-S7前脂肪细胞和人原代前脂肪细胞分别以密度1×105和2.5×105接种到24孔板中。过夜培养后，培养基补充有DHEA，雄烯二醇或DHEAS(0-100μM)。孵育24，48或72小时后，通过与放射性标记的胸苷(0.2μCi/孔)一起温育培养最后6小时来评估细胞增殖。用TCA沉淀蛋白质，并在NaOH中刮擦细胞。通过闪烁计数分析所得裂解物中放射性标记的核材料的相应含量。数据表示为对照的百分比[2]。 |
| Animal Experiment | 小鼠[3]给小鼠喂食Purina Lab Chow直到实验开始(第0天)。然后在0900和1000小时之间喂食5只小鼠的组，其中含有无添加剂或DHEA(0.45％w/w)的沉淀的AIN-76A饮食。饮食在4°C下储存不超过六个月，以保持最佳活动。除了对用DHEA处理的小鼠配对喂养的小鼠外，小鼠随意给予饮食。饲喂配对喂养的小鼠的AIN-76A饮食量由每天喂食DHEA的小鼠消耗的食物重量决定。从第1天开始到第59天结束的不同时间点测量体重(克)。通过称重每只5只小鼠的食物消耗来确定每日食物摄入量(克/天)。计算第1周至第8周的平均值±SEM值(n = 7);第9周只有3天。 |
| Kinase Experiment | 将Chub-S7细胞在含有冷DHEA(20nM)和氚化DHEA(0.2μCi/孔)的DMEM中孵育48小时。温育后，使用二氯甲烷萃取类固醇，使用正己烷/ 1-己醇(75:25)作为流动相系统，通过薄层色谱法分离。通过与未标记的参考类固醇的迁移来鉴定代谢物，所述类固醇通过暴露于Lieberman-Burchard试剂(乙醇 - 乙酸酐 - 硫酸)可视化。使用LabLogic AR-200扫描仪量化类固醇转化。使用比色96孔板测定法测量蛋白质浓度并用于标准化转化。活动表示为转化百分比[2]。 |
| Data Literature Source | [1]. Anagnostopoulou V，et al. Differential effects of dehydroepiandrosterone and testosterone in prostate and colon cancer cell apoptosis: the role of nerve growth factor (NGF) receptors. Endocrinology. 2013 Jul;154(7):2446-56. [2]. McNelis JC，et al. Dehydroepiandrosterone exerts anti-glucocorticoid action on human preadipocyte proliferation，differentiation and glucose uptake. Am J Physiol Endocrinol Metab. 2013 Nov 1;305(9):E1134-44. [3]. Catalina F，et al. Decrease of core body temperature in mice by dehydroepiandrosterone. Exp Biol Med (Maywood). 2002 Jun;227(6):382-8. |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg |
DHEA is one of the most abundant steroid hormones. DHEA mediates its action through a variety of signaling pathways and acts through its specific receptors through conversion into androgens and estrogen derivatives (e.g., androgens, estrogens, 7α and 7β DHEA, and 7α and 7β epiandrosterone derivatives). It is an important endogenous steroid hormone that acts as an androgen receptor antagonist and an estrogen receptor agonist.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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