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My Cart> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > Endocrinology & Hormones > DexamethasoneCAS：1950/2/2
Molecular Formula：C22H29FO5
Molecular Weight：392.46
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
Qty：
Size：
| CAS | 1950/2/2 |
| Name | Dexamethasone |
| Molecular Formula | C22H29FO5 |
| Molecular Weight | 392.46 |
| Solubility | Soluble in DMSO ≥50mg/mL;Soluble in Ethanol ≥5mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 200-003-9 |
| MDL | MFCD00064136 |
| SMILES | O=C1C=C[C@@]2(C)C(CC[C@]3([H])[C@]2(F)[C@@H](O)C[C@@]4(C)[C@@]3([H])C[C@@H](C)[C@@]4(C(CO)=O)O)=C1 |
| InChIKey | UREBDLICKHMUKA-CXSFZGCWSA-N |
| InChI | InChI=1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1 |
| PubChem CID | 5743 |
| Target Point | Glucocorticoid Receptor (GR, or GCR);interleukin receptor |
| Passage | Endocrinology & Hormones;Immunology & Inflammation |
| Background | Dexamethasone is a glucocorticoid receptor agonist with anti-inflammatory and immunosuppressive effects. |
| Biological Activity | Dexamethasone 是一种糖皮质激素受体 (glucocorticoid receptor) 激动剂。Dexamethasone调节几种转录因子，包括激活蛋白-1，核因子-AT和核因子-κB，导致激活和抑制参与炎症反应的关键基因[1-5]。 |
| In Vitro | 地塞米松有效抑制A549细胞释放粒细胞 - 巨噬细胞集落刺激因子(GM-CSF)，EC50为2.2 nM。发现地塞米松(EC50 = 36nM)诱导β2受体的转录与糖皮质激素受体(GR)DNA结合相关，并且发生在比GM-CSF释放抑制高10-100倍的浓度。地塞米松(IC50 = 0.5 nM)抑制3×κB(NF-κB，IκBα和I-κBβ)，这与抑制GM-CSF释放有关[2]。 |
| In Vivo | 先前已报道用2×5mg/kg剂量的地塞米松有效抑制脂多糖(LPS)诱导的炎症。在我们的实验系统中，与暴露于LPS并仅注射溶剂(盐水)的动物相比，用单剂量地塞米松10mg/kg(ip)治疗显著降低了粒细胞的募集以及氧自由基的自发产生。当在吸入LPS之前1小时和之后1小时施用时，效果是统计学显著的。 BALF中的粒细胞数量下降到与健康动物相当的水平(给定水雾剂)[3]。用地塞米松处理的大鼠比对照大鼠消耗更少的食物并且重量更轻。虽然他们的食物摄入量相似，但处理过的大鼠也比配对喂养的动物重量轻。 5天的地塞米松注射导致肝脏质量(+ 42％)和肝脏与体重比率(+ 65％)显著增加。治疗5天后，腓肠肌的湿重减少20％，但相对于体重(g/100 g体重)，它仍未受影响，表明肌肉减重与体重减轻平行[4]。 |
| Animal Experiment | 小鼠[3]雌性C57Bl/6JBom小鼠(10-12周龄)用于所有实验。地塞米松以1或10mg/kg的单次注射给药。将地塞米松溶于盐水中，在LPS暴露前1小时或1小时腹膜内注射400μL。在一个实验中，从攻击前1小时开始每4 5小时连续注射N-乙酰半胱氨酸(NAC)(100和500mg/kg)(总共5次注射)。对照组的LPS暴露的动物腹膜内注射溶剂(盐水)。通过将100μLNAAC(50，100或500mg/kg)或地塞米松(10mg/kg)滴注到用15mg/kg Rapinovet(iv)麻醉的小鼠的肺中来进行气管内给药。使用大鼠[4]雄性Sprague-Dawley大鼠。每天一次用地塞米松(1.5mg/kg体重)腹膜内注射地塞米松处理的大鼠5天，并允许随意喂食。特别选择地塞米松剂量(1.5mg/kg /天)和治疗持续时间(5天)，因为该治疗诱导了可重复且显著的分解代谢状态。对照大鼠未接受任何治疗并随意喂食。为了考虑地塞米松处理诱导的食物摄入减少，使用第三组配对喂养的大鼠。给这些大鼠提供与注射地塞米松的大鼠相同量的食物，并用每日等容的腹膜内注射NaCl(0.9％)处理5天。在最后注射地塞米松或NaCl后，将动物禁食过夜，然后通过断头处死。 |
| Data Literature Source | [1]. LaLone CA，et al. Effects of a glucocorticoid receptor agonist，Dexamethasone，on fathead minnow reproduction，growth，and development. Environ Toxicol Chem. 2012 Mar;31(3):611-22. [2]. Adcock IM，et al. Ligand-induced differentiation of glucocorticoid receptor (GR) trans-repression and transactivation: preferential targetting of NF-kappaB and lack of I-kappaB involvement. Br J Pharmacol. 1999 Jun;127(4):1003-11 [3]. Rocksén D，et al. Differential anti-inflammatory and anti-oxidative effects of Dexamethasone and N-acetylcysteine in endotoxin-induced lung inflammation. Clin Exp Immunol. 2000 Nov;122(2):249-56 [4]. Roussel D，et al. Dexamethasone treatment specifically increases the basal proton conductance of rat liver mitochondria. FEBS Lett. 2003 Apr 24;541(1-3):75-9. [5]. Maher HM，et al. Simultaneous determination of selected tyrosine kinase inhibitors with corticosteroids and antiemetics in rat plasma by solid phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry: Application to pharmacokinetic interaction studies. J Pharm Biomed Anal. 2016 May 30;124:216-27. |
| Unit | Bottle |
| Specification | 100mg 10mM*1mL in DMSO 500mg |
Dexamethasone is a basic agent for osteogenesis induction, which can activate glucocorticoid receptors located on the surface of stem cells and significantly increase the activity of alkaline phosphatase. It can induce stem cells to differentiate into osteoblasts.
This product can be used for induction of osteoblasts and other cells.
Examples of using this product（for reference only）
In Vitro:
Cell（1 μM dexamethasone/adipogenic； 10 nM dexamethasone/Osteogenic）：
The adipogenic medium was the complete culture medium supplemented with 1 μM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/L insulin (Solarbio), and 0.5 mM isobutyl-methylxanthine (Solarbio).The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio).
Reference：
Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.
Cell（Osteogenic；100 nM dexamethasone）：
Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining.
Reference：
Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421.
Cell (Osteogenic：10 nM dexamethasone; adipogenic： 1 mM dexamethasone )：
Osteogenic differentiation assay：
Cells were cultured in the osteogenic medium which contained 90% a-MEM (BI), 10% FBS (BI), 10 nM dexamethasone (Solarbio,Beijing, China), 10 mM b-glycerophosphate (Solarbio) and 50 mg/L ascorbic acid (Solarbio).
Adipogenic differentiation assay：
Cells were cultured in the adipogenic medium which contained 90% a-MEM (BI), 10% FBS (BI), 1 mM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/L insulin (Solarbio) and 0.5 mM isobutyl-methylxanthine (Solarbio).
Reference：
Jia L, Zhang Y, Ji Y, Xiong Y, Zhang W, Wen Y, Xu X. YAP balances the osteogenic and adipogenic differentiation of hPDLSCs In Vitro partly through the Wnt/β-catenin signaling pathway. Biochem Biophys Res Commun. 2019 Oct 8;518(1):154-160. doi: 10.1016/j.bbrc.2019.08.024. Epub 2019 Aug 14. PMID: 31421821.
Cell（BMSCs and HUVECs；10 μM or 100 μM dexamethasone/DEX）：
The cells were used between the third and sixth passages (p3–p6) and divided into six groups for the cell counting kit-8 (CCK-8) assay: (1) control; (2) dexamethasone (DEX; 10 μM; Solarbio Co., Ltd.; concentration was selected based on previous studies); (3) DEX + 5 μM AS-IV; (4) DEX + 20 μM AS-IV; (5) DEX + 50 μM AS-IV; and (6) DEX + 100 μM AS-IV. For alizarin red staining; alkaline phosphatase (ALP) staining; and apoptosis, tube formation, scratch wound and Transwell migration assays, the cells were divided into control, DEX, DEX + AS-IV and DEX + AS-IV + LY294002 groups. DEX concentration was 10 μM for alizarin red staining; ALP staining; and tube formation, Transwell migration and scratch wound assays; and 100 μM for the apoptosis assay. AS-IV and DEX were administered simultaneously. The concentrations of AS-IV and LY294002 were 50 and 10 μM, respectively.
Reference：
Shan H, Lin Y, Yin F, Pan C, Hou J, Wu T, Xia W, Zuo R, Cao B, Jiang C, Zhou Z, Yu X. Effects of astragaloside IV on glucocorticoid-induced avascular necrosis of the femoral head via regulating Akt-related pathways. Cell Prolif. 2023 Apr 26:e13485. doi: 10.1111/cpr.13485. Epub ahead of print. PMID: 37186483.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
JWu, et al. European Journal of Pharmacology, 2019, 859:172484.
Shan H, et al. Cell Prolif. 2023 Apr 26:e13485.
Shan H, et al. Cell Prolif. 2023 Apr 26:e13485.
Sorry, there is no more information.
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能溶于水吗 2023-09-25 15:11:01
AdministratorDexamethasone地塞米松不溶于水。