{{cart_num}}
My CartCAS：458-37-7
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Light yellow to yellow Solid
Qty：
Size：
| CAS | 458-37-7 |
| Name | Curcumin |
| Molecular Formula | C21H20O6 |
| Molecular Weight | 368.38 |
| Solubility | Soluble in DMSO ≥10mg/mL |
| Purity | HPLC≥98% |
| Appearance | Light yellow to yellow Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 207-280-5 |
| MDL | MFCD00008365 |
| SMILES | O=C(CC(/C=C/C1=CC=C(O)C(OC)=C1)=O)/C=C/C2=CC=C(O)C(OC)=C2 |
| InChIKey | VFLDPWHFBUODDF-FCXRPNKRSA-N |
| InChI | InChI=1S/C21H20O6/c1-26-20-11-14(5-9-18(20)24)3-7-16(22)13-17(23)8-4-15-6-10-19(25)21(12-15)27-2/h3-12,24-25H,13H2,1-2H3/b7-3+,8-4+ |
| PubChem CID | 969516 |
| Target Point | P300/CREB;Histone acetyltransferase (HAT);Keap1-Nrf2 |
| Passage | Epigenetics;NF-κB |
| Background | Curcumin is a specific inhibitor of acetyltransferase p300/CREB binding protein that inhibits histone/non-histone acetylation and histone acetyltransferase-dependent chromatin transcription. Curcumin can inhibit NF-κb and MAPKs, and has anti-inflammatory, antioxidant, anti-angiogenesis and other physiological activities. |
| Biological Activity | Curcumin是一种天然酚类化合物，具有抗炎，抗氧化，抗增殖和抗血管生成等多种活性。 Curcumin是p300组蛋白乙酰转移酶 ((HATs)) 抑制剂，也显示对 NF-κB 和MAPKs 的抑制作用。[1-6] |
| In Vitro | 姜黄素部分通过激活核因子(红细胞-2相关)因子2(Nrf2)及其抗氧化剂和II期解毒酶发挥其化学预防作用[1]。姜黄素抑制T47D细胞生长，分别为24，48和72小时MTT测定的IC50为25，19和17.5μM。姜黄素和水飞蓟宾混合物对T47D细胞的IC50分别为17.5，15和12μM，暴露时间分别为24，48和72小时[2]。姜黄素(2.5-80μM)诱导AGS和HT-29细胞系中的凋亡细胞死亡，并且在AGS和HT-29细胞系中IC50分别为21.9±0.1，40.7±0.5μM。姜黄素诱导的细胞凋亡需要AGS和HT-29细胞中的半胱天冬酶活性。姜黄素诱导ER Ca2 +下降和线粒体Ca2 +超载[3]。姜黄素以剂量依赖性方式诱导LNCaP和PC-3细胞的G2/M细胞周期停滞。姜黄素上调NF-κB抑制剂IkappaBalpha的蛋白质水平，下调c-Jun和AR的蛋白质水平[5]。 |
| In Vivo | 与暴露于CMS的大鼠相比，姜黄素(10mg/kg，po)显著地防止了蔗糖消耗百分比的降低。姜黄素治疗可显著预防应激大鼠TNF-α和IL-6水平的升高[4]。姜黄素以20mg/kg(ip)降低p300/CREB结合蛋白(CBP)在脑源性神经营养因子(BDNF)启动子上的结合，降低40mg/kg的BDNF启动子处P300/CBP的结合，和在慢性压迫性损伤(CCI)大鼠中，在60mg/kg的BDNF启动子处，p300/CBP和H3K9ac/H4K5ac的所有四种蛋白质均降低[6]。 |
| Cell Experiment | T47D乳腺癌细胞系在补充有10％FBS，2mg/mL碳酸氢钠，0.05mg/mL青霉素G，0.08mg/mL链霉素的RPMI 1640中生长。将培养物保持在塑料烧瓶上并在37℃，5％CO 2中温育。在培养足够量的细胞后，通过24，48和72小时MTT测定研究水飞蓟宾和姜黄素的细胞毒性作用，其中在96孔板中培养1000个细胞/孔。在37°C和含5％CO2的潮湿气氛中孵育24小时后，用连续浓度的姜黄素(5，10，20，30，40，50，60，80，100μM)，水飞蓟宾(20，40，60，80，100，120，140，180，200μM)和姜黄素 - 水飞蓟宾混合物(每种都有5，10，20，30，40，50，60，80，100μM)24，48除了含有200μL含有10％DMSO的培养基的细胞用于对照之外，以一式四份的方式72小时和72小时。温育后，将平板的所有孔的培养基更换为新鲜培养基，并将细胞在培养箱中放置24小时。然后，小心地除去所有孔的培养基，并向每个孔中加入溶解在PBS中的50μL2mg/ mL MTT，并用铝箔覆盖该板并再次温育4.5小时。除去孔的内容物后，向孔中加入200μL纯DMSO。然后，加入25μLSorensen的甘氨酸缓冲液，并使用参考波长为630nm的EL×800微孔板吸光度读数器在570nm读取每个孔的吸光度。 |
| Animal Experiment | 将新鲜悬浮于盐水中的姜黄素(10mg/kg)通过口服强饲法每天一次施用，持续3周。将40只大鼠随机分为4组(n = 10 /每组)：组I接受生理盐水作为对照，组II接受姜黄素，组III接受CMS和接受盐水，组IV接受CMS和接受姜黄素。 |
| Data Literature Source | [1]. Gao S，et al. Curcumin attenuates arsenic-induced hepatic injuries and oxidative stress in experimental mice through activation of Nrf2 pathway，promotion of arsenic methylation and urinary excretion. Food Chem Toxicol. 2013 Jul 18. pii: S0278-6915(13)004 [2]. Nasiri M，et al. Curcumin and Silibinin Inhibit Telomerase Expression in T47D Human Breast Cancer Cells. Asian Pac J Cancer Prev. 2013;14(6):3449-53. [3]. Cao A，et all. Curcumin induces apoptosis in human gastric carcinoma AGS cells and colon carcinoma HT-29 cells through mitochondrial dysfunction and endoplasmic reticulum stress. Apoptosis. 2013 Jul 24. [Epub ahead of print] [4]. Jiang H，et al. Antidepressant-like effects of curcumin in chronic mild stress of rats: Involvement of its anti-inflammatory action. Prog Neuropsychopharmacol Biol Psychiatry. 2013 Jul 20. pii: S0278-5846(13)00150-4. [5]. Guo H，et al. Curcumin induces cell cycle arrest and apoptosis of prostate cancer cells by regulating the expression of IkappaBalpha，c-Jun and androgen receptor. Pharmazie. 2013 Jun;68(6):431-4. [6]. Zhu X，et al. Curcumin alleviates neuropathic pain by inhibiting p300/CBP histone acetyltransferase activity-regulated expression of BDNF and cox-2 in a rat model. PLoS One. 2014 Mar 6;9(3):e91303. |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg |
Curcumin是一种酚类化合物，是乙酰转移酶 p300/CREB 结合蛋白 特异性抑制剂，抑制组蛋白/非组蛋白的乙酰化和组蛋白乙酰转移酶依赖的染色质转录。Curcumin 对 NF-κb 和 MAPKs 有抑制作用，并具有抗炎、抗氧化、抗增殖和抗血管生成等多种生理活性。Curcumin 通过 Keap1 半胱氨酸修饰诱导 Nrf2 蛋白的稳定。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
Jia W, et al. Biomed Pharmacother. 2019 Mar;111:1204-1212.
Zhang Z, et al. Biomed Res Int. 2019 Oct 31;2019:8912961.
Manual Download
