Cisplatin
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Cat.No：IC0440 Solarbio
CAS：15663-27-1
Storage：Powder:2-8℃，2 years
Purity：HPLC≥98%
Appearance：Light yellow to yellow Solid
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> Small Molecule Compounds > Inhibitors & Antagonists & Agonists > DNA Damage & DNA Repai > Cisplatin
Product Information
| CAS | 15663-27-1 |
| Name | Cisplatin |
| Molecular Formula | Cl2H6N2Pt |
| Molecular Weight | 300.05 |
| Solubility | Soluble in DMF(This product is not stable in solution, it is recommended that you use it now) |
| Purity | HPLC≥98% |
| Appearance | Light yellow to yellow Solid |
| Storage | Powder:2-8℃，2 years |
| EC | EINECS 239-733-8 |
| MDL | MFCD00011623 |
| SMILES | [Cl-][Pt+2]([Cl-])([NH3])[NH3] |
| InChIKey | DQLATGHUWYMOKM-UHFFFAOYSA-L |
| InChI | InChI=1S/2ClH.2H2N.Pt/h2*1H;2*1H2;/q;;2*-1;+4/p-2 |
| PubChem CID | 2767 |
| Target Point | Ferroptosis;DNA Crosslinker |
| Passage | Apoptosis |
| Background | Cisplatin cross-linking with DNA can cause DNA damage in cancer cells and can be used in the research of anti-tumor chemotherapy drugs. Cisplatin can activate ferroptosis and induce autophagy. |
| Biological Activity | Cisplatin 是一种抗肿瘤化疗药物，它与 DNA 交联引起癌细胞中DNA损伤[1-4]。 |
| In Vitro | 顺铂(50μM)在肾近端小管细胞(RPTC)中产生时间依赖性细胞凋亡，导致细胞收缩，半胱天冬酶3活性增加50倍，磷脂酰丝氨酸外化增加4倍，增加5倍和15倍分别在染色质浓缩和DNA亚倍体中[2]。顺铂以剂量依赖性方式引起HeLa细胞的凋亡，浓度为30μM顺铂导致24小时处理后大于90％的细胞群死亡。使用30μM浓度检查顺铂诱导的细胞凋亡的动力学。顺铂激活MEK/ERK信号通路，20和30μM顺铂，两者均导致显著的细胞凋亡，导致ERK的强烈激活[1]。 |
| In Vivo | 在携带黑色素瘤的小鼠中，顺铂(4 mg/kg BW)可减小实体瘤的大小和重量，HemoHIM补充顺铂可增加肿瘤大小和体重的减少[3]。与对照大鼠相比，顺铂给药导致肾重量显著增加，占总体重，尿量，血清肌酐和血尿素氮的百分比分别为132，315，797和556％[4]。 |
| Cell Experiment | 将HeLa和A549细胞维持在补充有10％胎牛血清，100单位青霉素和100μg链霉素/ mL的Dulbecco改良Eagle培养基中。将它们在37℃下在含有5％CO 2的加湿室中培养。为了诱导细胞凋亡，在顺铂(0-30μM)处理前1天将细胞接种在60-mm培养皿中[1]。 |
| Animal Experiment | 小鼠[3]将小鼠随机分成三组(对照组，顺铂和顺铂+ HemoHIM)，每组由20只小鼠组成。在初始注射顺铂之前3天，将B16F0黑素瘤(5×10 5个细胞/小鼠)接种到小鼠的皮下股骨左侧区域。在第0，7和14天(总共三次注射)以4mg/kg体重(BW)腹膜内注射顺铂。实验组用HemoHIM插管，终浓度为100mg/kgB.W.每天从第-1天到第16天，而对照组仅接受水。在初始注射顺铂后第17天，分别对每组的所有小鼠进行实验，以评估肿瘤重量或肿瘤大小。肿瘤大小计算如下：肿瘤大小= ab2/2，其中a和b分别是更大和更小的直径。将体重为200-250g的大鼠[4]雄性Sprague-Dawley大鼠随机分成4组，每组4或5只动物。第一组(对照)接受用于辣椒素(Cap)的载体(5％羧甲基纤维素钠溶液(CMC-Na)，5mL/kg体重，po)。第二组在5％CMC-Na(5 mL/kg)中接受Cap(10 mg/kg/d，po)，第三组接受5％CMC-Na连续6天注射顺铂(5 mg/kg in生理盐水溶液，ip)。第四组在顺铂注射(5mg/kg，腹膜内注射)后连续6天在5％CMC-Na中接受Cap(10mg/kg/d，po)。对于所有团体，每天两次给予Cap或车辆。使用来自我们的初步实验的数据选择所选的帽浓度和剂量施用方案而不诱导任何大鼠肠损伤。 |
| Data Literature Source | [1]. Wang X，et al. Requirement for ERK activation in cisplatin-induced apoptosis. J Biol Chem. 2000 Dec 15;275(50):39435-43. [2]. Cummings BS，et al. Cisplatin-induced renal cell apoptosis: caspase 3-dependent and -independent pathways. J Pharmacol Exp Ther. 2002 Jul;302(1):8-17. [3]. Park HR，et al. Enhanced antitumor efficacy of cisplatin in combination with HemoHIM in tumor-bearing mice. BMC Cancer. 2009 Mar 17;9:85. [4]. Shimeda Y，et al. Protective effects of capsaicin against cisplatin-induced nephrotoxicity in rats. Biol Pharm Bull. 2005 Sep;28(9):1635-8. |
| Unit | Bottle |
| Specification | 20mg 100mg 200mg 500mg |
Examples of using this product（for reference only）：
In Vivo：
Mice（4-6 week male BALB/C nude mice；5 mg/kg cisplatin，intraperitoneally administered，every week for 2 weeks）:
4-6 week male BALB/C nude mice were subcutaneously injected with 1×106 cells near the extremities of four limbs. When the mice developed palpable tumors, the mice were intraperitoneally administered with cisplatin (5 mg/kg; Solarbio Company) every week for 2 weeks. The size of the tumors was measured once every 3 days, six times in a row. All animals were sacrificed 25 days after inoculation, and the tumors were collected.The tumor tissues were photographed, and immunohistochemistry was performed.
Reference：
Gong L, Xiao M, He D, Hu Y, Zhu Y, Xiang L, Bao Y, Liu X, Zeng Q, Liu J, Zhou M, Zhou Y, Cheng Y, Zhang Y, Deng L, Zhu R, Lan H, Cao K. WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma. Front Oncol. 2020 Apr 24;10:461. doi: 10.3389/fonc.2020.00461. PMID: 32426268; PMCID: PMC7212426.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Liu H, et al. Breast Cancer Res. 2023 Apr 17;25(1):43.
Pan Y, et al. Microsyst Nanoeng. 2020 Mar 9;6:23.
Gong L, et al. Front Oncol. 2020 Apr 24;10:461.
Pan Y, et al. Biosens Bioelectron. 2019 Apr 1;130:344-351.
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