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My CartCAS：55-98-1
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 55-98-1 |
| Name | Busulfan |
| Molecular Formula | C6H14O6S2 |
| Molecular Weight | 246.3 |
| Solubility | Soluble in DMSO ≥15mg/mL |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 200-250-2 |
| MDL | MFCD00007562 |
| SMILES | CS(=O)(OCCCCOS(C)(=O)=O)=O |
| InChIKey | COVZYZSDYWQREU-UHFFFAOYSA-N |
| InChI | InChI=1S/C6H14O6S2/c1-13(7,8)11-5-3-4-6-12-14(2,9)10/h3-6H2,1-2H3 |
| PubChem CID | 2478 |
| Target Point | DNA Alkylator |
| Passage | DNA Damage/DNA Repair |
| Background | Busulfan is an effective alkylator. It is a bifunctional alkylating agent of bismethyl sulfonate and is a cell cycle non-specific compound. It disrupts the structure and function of DNA by alkylating guanines in cellular DNA. |
| Biological Activity | Busulfan 是一种有效的烷化剂 (alkylator)，具有化疗作用。[1-3] |
| In Vitro | Busulfan(120μM)通过Erk和p38 MAPK途径导致WI38细胞过早衰老，降低GSH并增加ROS产生，但NADPH氧化酶可抑制产生[1]。 |
| In Vivo | 白消安(40mg/kg，ip)增加细胞凋亡并降低小鼠的睾丸重量。与对照相比，Busulfan处理小鼠的睾丸表现出更高水平的pRB表达，抑制Rb磷酸化和PCNA表达[2]。 Busulfan(2.5，5.0 mg/kg，ip)在大鼠中以剂量依赖性方式导致早期出现持续性esturs。白消安(5.0 mg/kg)也会增加子宫腺癌的发病率和子宫肿瘤病变的多样性。此外，Busulfan降低大鼠血清17β-雌二醇(E2)，孕酮和抑制素水平，并且仅在5.0 mg/kg时提高E2 /孕酮比例[3]。 |
| Cell Experiment | 将WI38细胞以60，000个细胞/孔接种于24孔板的孔中并培养过夜。用Busulfan处理它们24小时，然后除去药物并将细胞在新鲜培养基中培养最多11天。未处理的细胞用作对照。每3天对细胞进行胰蛋白酶消化，计数并在1：5和1：2稀释的新鲜培养基中进行传代培养，分别用于对照未处理和经Busulfan处理的细胞[1]。 |
| Animal Experiment | 小鼠[2]使用120只年龄为8至12周(30-40g)的ICR雄性小鼠。 8周龄ICR雄性小鼠接受单次腹膜内注射用芝麻油稀释的Busulfan(40mg/kg)。将小鼠置于22±1℃的线笼中，12小时光暗循环，70％湿度，随意喂食[2]。 |
| Data Literature Source | [1]. Probin V，et al. Busulfan-induced senescence is dependent on ROS production upstream of the MAPK pathway. Free Radic Biol Med. 2007 Jun 15;42(12):1858-65. Epub 2007 Mar 31. [2]. Choi YJ，et al. Murine male germ cell apoptosis induced by busulfan treatment correlates with loss of c-kit-expression in a Fas/FasL- and p53-independent manner. FEBS Lett. 2004 Sep 24;575(1-3):41-51. [3]. Yoshida M，et al. Reduction of primordial follicles caused by maternal treatment with busulfan promotes endometrial adenocarcinoma development in donryu rats. J Reprod Dev. 2005 Dec;51(6):707-14. Epub 2005 Sep 22. |
| Unit | Bottle |
| Specification | 100mg 10mM*1mL in DMSO |
Busulfan 是一种有效的烷化剂 (alkylator)。属双甲基磺酸酯类双功能烷化剂，为细胞周期非特异性化合物。通过与细胞DNA内的鸟嘌呤起烷化作用而破坏DNA的结构与功能。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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