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My CartCAS：59-14-3
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥99%
Appearance：White to off-white Solid
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| CAS | 59-14-3 |
| Name | 5-BrdU |
| Molecular Formula | C9H11BrN2O5 |
| Molecular Weight | 307.1 |
| Solubility | Soluble in DMSO ≥50mg/mL |
| Purity | HPLC≥99% |
| Appearance | White to off-white Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 200-415-9 |
| MDL | MFCD00006529 |
| SMILES | OC[C@@H]1[C@H](C[C@H](N2C(NC(C(Br)=C2)=O)=O)O1)O |
| InChIKey | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
| InChI | InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1 |
| PubChem CID | 6035 |
| Target Point | DNA/RNA Synthesis |
| Passage | Cell Cycle;DNA Damage/DNA Repair |
| Background | 5-BrdU is a nucleoside analog that competes with thymidine for incorporation into DNA. |
| Biological Activity | 5-BrdU是一种核苷类似物，可与胸苷竞争掺入DNA。 5-BrdU通常用于检测增殖细胞。[1-2] |
| In Vitro | 溴脱氧尿苷诱导癌细胞系和癌干细胞群扩增RG2大鼠神经胶质瘤细胞的进行性，剂量响应性抑制。在H9细胞和BJ成纤维细胞中，溴脱氧尿苷改变细胞周期谱[1]。 BrdU稳定整合到DNA中，因此可用于评估细胞增殖和其他细胞过程[2]。 |
| In Vivo | Bromodeoxyuridine(300 mg/kg，ip或0.8 mg/mL，po)显著减缓大鼠胶质瘤RG2肿瘤模型中的肿瘤进展[1]。 |
| Cell Experiment | 最初以2000个细胞/ cm 2接种培养物，并用Z2 Coulter计数器定量。将RG2大鼠神经胶质瘤细胞用0，1，10或50μMBrdU处理24小时，并在18天内获得累积生长曲线。对照和处理的细胞进行定量，并在处理后第5，12和18天以相同的密度重新铺板。 |
| Data Literature Source | [1]. Levkoff LH，et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16. [2]. Rothaeusler K，et al. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31 |
| Unit | Bottle |
| Specification | 100mg 10mM*1mL in DMSO 500mg |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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Shao L, et al. Stem Cell Res Ther. 2018 Aug 14;9(1):219.
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