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My CartCAS：590-46-5
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：White to off-white Solid
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| CAS | 590-46-5 |
| Name | Betaine Hydrochloride |
| Molecular Formula | C5H11NO2·HCl |
| Molecular Weight | 153.61 |
| Solubility | Soluble in Water/DMSO |
| Purity | HPLC≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 209-683-1 |
| MDL | MFCD00011903 |
| SMILES | C[N+](C)(C)CC(O)=O.[Cl-] |
| InChIKey | HOPSCVCBEOCPJZ-UHFFFAOYSA-N |
| InChI | InChI=1S/C5H11NO2.ClH/c1-6(2,3)4-5(7)8;/h4H2,1-3H3;1H |
| PubChem CID | 11545 |
| Target Point | Others |
| Passage | Others |
| Background | Betaine Hydrochloride is an active methyl donor that maintains normal DNA methylation patterns. |
| Biological Activity | Betaine hydrochloride 是许多食物中发现的天然化合物，也是一种可维持正常 DNA 甲基化模式的活性甲基供体。[1-2] |
| In Vitro | 发现随着MTT试验暴露于增加的甜菜碱盐酸盐水平，HeLa细胞的增殖被显著抑制(p <0.05)。低剂量组(<5 mg / mL)中S期细胞的百分比明显高于高剂量组，而Sub-G1期的比例则相反(p <0.05)。高浓度的甜菜碱盐酸盐(> 5.0 mg / mL)显著促进HeLa细胞的凋亡(p <0.01)。低剂量组SOD活性略高于对照组(p <0.05)，促凋亡基因Bax，P53，Caspase 3和凋亡抑制基因Bcl-2的表达存在明显的同步性和相关性。响应细胞凋亡诱导刺激，p53和细胞周期蛋白D1可以在G1 / S或S / G2检查点阻断细胞周期而被激活[1]。 |
| In Vivo | 补充甜菜碱盐酸盐可减轻肝脏与体重的比例。当在含酒精的液体饮食中加入甜菜碱盐酸盐时，肝脏甘油三酯(TG)积聚和肝损伤均显著降低。甜菜碱盐酸盐补充减少慢性酒精摄入引起的附睾脂肪垫质量损失。甜菜碱盐酸盐补充剂可防止S-腺苷同型半胱氨酸(SAM)的消耗，并减少S-腺苷同型半胱氨酸(SAH)的增加，从而改善脂肪组织的甲基化状态[2]。 |
| Cell Experiment | 通过MTT测定评估盐酸甜菜碱的细胞毒性作用。简而言之，将5×103 HeLa或MCF-10A细胞/孔接种在96孔板中并培养过夜。然后，向每个孔中加入不同浓度的甜菜碱盐酸盐(0至100mg/mL)，并在孵育后24，48，72和96小时进行MTT测定。根据制造商的说明[1]，使用超氧化物歧化酶测定试剂盒检测Cu/Zn-SOD活性。 |
| Animal Experiment | 在该研究中使用重25±0.5g的雄性C57BL/6小鼠。将动物随机分配到四组(每组n = 6至8)，并且通过逐步进食程序将改良的Lieber-DeCarli酒精(AF)或等热量麦芽糖糊精对照液体饮食(PF)配对喂养5周。乙醇含量从总热量的30％开始，并在上周逐渐增加至36％。甜菜碱盐酸盐作为补充剂在液体饮食(PF和AF)中以0.5％(w/v)的浓度与醇同时施用。所有动物都可随意饮食和饮水。在实验结束时，小鼠在被剥夺食物4小时后被杀死，并且收集血浆，肝脏和附睾脂肪垫样品用于测定[2]。 |
| Data Literature Source | [1]. Guo Y，et al. Betaine Effects on Morphology，Proliferation，and p53-induced Apoptosis of HeLa Cervical Carcinoma Cells in Vitro. Asian Pac J Cancer Prev. 2015;16(8):3195-201. [2]. Dou X，et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014 Sep;171(17):4073-86 |
| Unit | Bottle |
| Specification | 20mg 10mM*1mL in DMSO 10mM*1mL in Water 100mg |
是一种可维持正常 DNA 甲基化模式的活性甲基供体。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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