{{cart_num}}
My CartCAS：520-36-5
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：HPLC≥98%
Appearance：Light yellow to yellow Solid
Qty：
Size：
| CAS | 520-36-5 |
| Name | Apigenin |
| Molecular Formula | C15H10O5 |
| Molecular Weight | 270.24 |
| Solubility | Soluble in DMSO ≥10mg/mL |
| Purity | HPLC≥98% |
| Appearance | Light yellow to yellow Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 208-292-3 |
| MDL | MFCD00006831 |
| SMILES | OC1=C2C(OC(C3=CC=C(O)C=C3)=CC2=O)=CC(O)=C1 |
| InChIKey | KZNIFHPLKGYRTM-UHFFFAOYSA-N |
| InChI | InChI=1S/C15H10O5/c16-9-3-1-8(2-4-9)13-7-12(19)15-11(18)5-10(17)6-14(15)20-13/h1-7,16-18H |
| PubChem CID | 5280443 |
| Target Point | Cytochrome P450 |
| Passage | Metabolic Enzyme&Protease |
| Background | Apigenin is a competitive CYP2C9 inhibitor. |
| Biological Activity | Apigenin 是一种竞争性 CYP2C9 抑制剂，Ki 为 2 μM。[1-3] |
| IC50 | Ki: 2μM(CYP2C9)[1] |
| In Vitro | 芹菜素在CYP2C9 RECO系统(含有重组人CYP2C9，P450还原酶，细胞色素b5和脂质体的纯化重组酶系统)中抑制细胞色素P450 2C9(CYP2C9)，Ki为2μM[1]。芹菜素抑制细胞增殖。在第7天，20，40和80μM芹菜素的生长抑制率(IR)分别为38％，71％和99％。在暴露于芹菜素24或48小时后，以剂量和时间依赖性方式抑制SGC-7901细胞的克隆形成。用芹菜素处理24和48小时后，80μM的克隆效率分别为9.8％和5％，而对照组则为40.4％和43.4％[2]。 |
| In Vivo | 芹菜素(API)是一种天然黄酮类化合物，具有广谱的生物学特性，包括抗氧化，抗炎，抗癌和神经保护作用。芹菜素(125mg/kg和250mg/kg)减轻阿霉素(ADR)(24mg/kg)诱导的心肌损伤。芹菜素抑制血清天冬氨酸氨基转移酶(AST)的释放。芹菜素降低血清乳酸脱氢酶(LDH)释放。芹菜素降低血清肌酸激酶(CK)含量[3]。 |
| Cell Experiment | 通过MTT测定确定芹菜素对细胞活力的影响。用0.2％EDTA收获人胃癌SGC-7901细胞的近汇合原种培养物，并以2.5×103 /孔的密度接种在96孔微量滴定板中。孵育过夜以允许细胞附着后，用含有不同浓度(0，20，40和80μM)芹菜素的新鲜培养基替换培养基。对照孔接受DMSO(0.2％)。在4个孔中重复各种浓度的芹菜素。温育24小时后，用酶标仪在570nm波长下测定一个平板。在测定之前，将MTT(PBS中5mg/mL)加入到每个孔中并孵育4小时，然后通过抽吸从孔中除去MTT溶液。小心地除去培养基后，向每个孔中加入0.1mL DMSO，并将板振荡15分钟。将7d的数据输入计算机并绘制生长曲线。计算生长抑制率(IR)[2]。 |
| Animal Experiment | 小鼠[3]将60只健康昆明小鼠(26±2g)随机分为两组：对照组(n = 15)和ADR组(n = 45)。 ADR组分为三个亚组：仅不含芹菜素(ADR，n = 15)的ADR，低剂量芹菜素(125 mg/kg /天，n = 15)和高剂量芹菜素(250 mg/kg /天)，n = 15)。所有芹菜素处理组每天通过胃管饲法用125或250mg/kg /天剂量治疗17天。将ADR(3mg/kg /天)以48小时的间隔腹膜内注射到动物体内(总共8次，累积剂量为24mg/kg)。对照组小鼠接受0.9％无菌盐水注射。在第一次处理后第17天，处死小鼠，并收集血样。用2.5％戊二醛固定剂固定许多心脏用于电子显微镜分析，并将其它心脏储存在-80℃用于蛋白质印迹分析。 |
| Data Literature Source | [1]. Si D，et al. Mechanism of CYP2C9 inhibition by flavones and flavonols. Drug Metab Dispos. 2009 Mar;37(3):629-34. [2]. Wu K，et al. Inhibitory effects of apigenin on the growth of gastric carcinoma SGC-7901 cells. World J Gastroenterol. 2005 Aug 7;11(29):4461-4. [3]. Yu W，et al. Apigenin Attenuates Adriamycin-Induced Cardiomyocyte Apoptosis via the PI3K/AKT/mTOR Pathway. Evid Based Complement Alternat Med. 2017;2017:2590676 |
| Unit | Bottle |
| Specification | 10mg 10mM*1mL in DMSO 50mg 100mg |
是一种竞争性 CYP2C9 抑制剂。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no more information.
Manual Download
