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My CartCAS：30931-67-0
Storage：Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：Light green to green Solid
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| CAS | 30931-67-0 |
| Name | ABTS Diammonium Salt |
| Molecular Formula | C18H24N6O6S4 |
| Molecular Weight | 548.68 |
| Solubility | Soluble in Water ≥50mg/mL;Soluble in DMSO ≥6mg/mL |
| Purity | ≥98% |
| Appearance | Light green to green Solid |
| Storage | Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 250-396-6 |
| MDL | MFCD00010404 |
| SMILES | CCN1C(C=CC(S(=O)([O-])=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)([O-])=O)=C3)\N4CC.[NH4+].[NH4+] |
| InChIKey | OHDRQQURAXLVGJ-AXMZSLBLSA-N |
| InChI | InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;; |
| PubChem CID | 16240279 |
| Background | ABTS Diammonium Salt is a substrate for horseradish peroxidase (HRP). |
| Biological Activity | ABTS diammonium salt 是一种辣根过氧化物酶 (HRP) 的底物。[1-2] |
| In Vitro | 研究了使用ABTS作为HRP缀合物底物的酶联免疫吸附测定(ELISA)的微技术。在4个底物的比较研究中，即; 5-氨基水杨酸(5AS)，邻苯二胺(OPD)，O-联甲苯胺(OT)和ABTS，对于HRP的敏感性，ABTS是最敏感，最稳定，最具视觉效果的蓝绿色[ 1]。 ABTS是典型的过氧化物酶底物。为了纯化和表征，过氧化物酶阳性转化体在培养上清液中产生活性蛋白的条件下大规模培养(XL)。培养160小时后，获得相对于底物ABTS的55，000U / L的活性，并收获含有过氧化物酶的上清液。以ABTS为底物，当H2O2浓度超过0.125mM时，过氧化物酶活性显着下降，表明该酶被H2O2抑制。最大反应速率取决于测试的底物，其值在31.2和125μM之间[2]。 |
| Kinase Experiment | 使用温度控制的多模板读数器或者在UV / Vis分光光度计中光度测定酶活性。通过添加酶引发反应。在25℃，在适当的基质波长下，在10分钟内测量酶活性。一个单位(1U)定义为每分钟转化1μmol底物的酶量。各种H2O2浓度(0-1250μM，酶浓度(0.27-54 nM)和底物浓度)用于测定酶活性。在pH3.8的100mM乙酸钠缓冲液和最终H2O2浓度下测定rPsaDyP对ABTS的活性。在420nm(ε42036，000Lmol-1 cm-1)下研究ABTS阳离子自由基的产生[2]。 |
| Data Literature Source | [1]. Matsuda H， et al. Evaluation of ELISA with ABTS， 2-2'-azino-di- (3-ethylbenzthiazoline sulfonic acid) ， as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun; 54 (3) :131-8. [2]. Lauber C， et al. Identification， heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23; 7 (1) :164 |
| Unit | Bottle |
| Specification | 10mM*1mL in DMSO 10mM*1mL in Water 500mg |
It is a substrate of horseradish peroxidase (HRP). ABTS is oxidized to form a stable blue-green cationic ABTS radical with maximum absorption peaks at 405 nm or 734 nm. After the tested substance is added to ABTS free radical solution, the antioxidant components can react with ABTS free radicals and make the reaction system fade, and the absorbance of 405 nm decreases, and the change of absorbance is proportional to the degree of free radical removal within a certain range. The absorbance drop was measured to reflect the ability of the sample to remove ABTS free radicals. ABTS method can be used to determine the antioxidant capacity of hydrophilic and lipophilic substances.
Examples of using this product（for reference only）
ABTS Free Radical Scavenging Assay.
1To be specific, ABTS (25 mg) was added to the potassium persulfate (6.5 mL, 2.45 mmol/L) solution. Subsequently, the prepared ABTS solution was stored (14 h) at a constant temperature (25 ℃).The maintenance of the absorbance (734 nm, 0.70 ± 0.10) was confirmed before the experiments. Then, fresh MPN-GAs (1.0 mL, 1 mg/mL) and the prepared ABTS (3 mL) solution were subjected to an incubation process (60 min). Final ABTS scavenging activities were evaluated through the transformation of measured absorbance (734 nm).
Reference：
Chen J, Chen X, Zhou G, Xu X. New insights into the ultrasound impact on covalent reactions of myofibrillar protein. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973. Epub 2022 Mar 3. PMID: 35272240; PMCID: PMC8913343.
2ABTS was dissolved in 25 mM Tris, pH 7.0, to a 7 mM concentration.
The ABTS radical cation (ABTS+·) was produced by mixing the ABTS stock solution with 2.45 mM potassium persulfate at a ratio of 1:1 (v:v) following 12?16 h of incubation in the dark (4 ℃). The solution was then diluted with Tris buffer to an absorbance of 0.70 (±0.02) at 734 nm to form the test reagent. The test sample (3 μL) was then mixed with 237 μL of the diluted ABTS solution. The mixture reacted against light at room temperature for 30 min, and the absorbance was recorded at 734 nm on a ultraviolet spectrometer (AE). The blank was made from 3 μL of Tris buffer and 237 μL of diluted ABTS solution (AB). The percentage of inhibition of ABTS+· was calculated using the formula:rate of inhibition=[ （AB-AE）/AB ] X 100where AB is the absorbance of the blank sample and AE is the absorbance of the mixtures.
Reference：
Ma J, Yao Q, Chen X, Lv C, Zang J, Zhao G. Weak Binding of Epigallocatechin to α-Lactalbumin Greatly Improves Its Stability and Uptake by Caco-2 Cells. J Agric Food Chem. 2021 Aug 4;69(30):8482-8491. doi: 10.1021/acs.jafc.1c03427. Epub 2021 Jul 21. PMID: 34286590.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Ma J, et al. Agric Food Chem. 2021 Aug 4;69(30):8482-8491. doi: 10.1021/acs.jafc.1c03427.
Chen J, et al. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973.
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