AO Stain Solution

Product Introduction：
DNA content is detected by flow cytometry, DNA is quantitatively analyzed by staining, and morphological changes are observed by fluorescence microscopy.
AO belongs to tricyclic aromatic fuel, which can label DNA and RNA, and belongs to metachromatic fluorescent dye. The dye is membrane-permeable and can penetrate the cell membrane to stain nuclear DNA and RNA. Therefore, AO is often used to detect intracellular DNA and RNA. The binding modes of AO and nucleic acid are as follows: 1. Intercalative binding, AO is embedded between the base pairs of nucleic acid double strands, and this binding mode is mainly the binding of AO and DNA. Its fluorescence emission peak is 530nm, and it is green fluorescence after excitation; 2, electrostatic attraction, with positive charge of AO and single-stranded nucleic acid phosphate (negative charge) to produce electrostatic attraction binding, this binding mode is mainly AO and RNA binding, its fluorescence emission peak is 640nm, after excitation is red fluorescence, a small amount of binding will be orange yellow or orange red fluorescence. Thus, AO chimeric into double-stranded DNA molecules appears green, and orange-yellow or orange-red fluorescence when bound to DNA single-stranded or RNA.
AO staining solution (1mg/ml) is used as storage solution and should be diluted to the appropriate concentration before use. Under the fluorescence microscope, AO could penetrate the normal cell membrane and make the nucleus appear green or yellow-green uniform fluorescence. However, in apoptotic cells, chromatin condensation or fragmentation can lead to the formation of apoptotic bodies. AO stained them with dense yellow-green fluorescence or yellow-green fragments. The yellow fluorescence of necrotic cells is weakened or even disappeared. AO staining is often combined with EB staining, because EB staining only stained dead cells and produced orange fluorescence, so that normal cells, apoptotic cells and necrotic cells could be distinguished.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

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