PYG Broth Base
Cat.No:LA5570 Solarbio
Storage:RT
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Name | PYG Broth Base |
Storage | RT |
Grade | BR |
Unit | Bottle |
Specification | 250g |
Product Introduction:
Used for bifidobacterium growth culture. One 2.5mg solution of heme chloride and one 1mg solution of vitamin K1 were added to every 100ml medium.
Preparation Method:
Weigh 18.04g of this product, heat it and dissolve it in 500ml distilled water, autoclave it at 121℃ for 15 minutes, cool it to about 50℃, add 0.5ml vitamin K1 solution filtered for sterilization and 2.5ml heme chloride solution (5mg/ml), mix well and set aside.
Composition: g/L
Peptone: 20 Glucose: 5.0
Yeast Infusion powder 10.0 Sodium chloride: 0.08
Calcium chloride: 0.008 Magnesium sulfate: 0.008
Potassium hydrogen phosphate: 0.04 sodium bicarbonate: 0.4
Cysteine hydrochloride 0.5 potassium dihydrogen phosphate 0.04pH: 6.0±0.1, 25℃
Precaution:
Preparation of vitamin K1 solution: weigh vitamin K11g, add anhydrous ethanol 99ml, filter and remove bacteria.
Preparation of heme chloride solution: Weigh 0.5g heme chloride and dissolve in 1mol/L sodium hydroxide solution 1ml plus distilled water to 100ml, autoclave at 121℃, and store in refrigerator.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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