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Application：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：3906.25 pg/ml
Storage：2-8℃，6 months
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| Name | Mouse IgG2b ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IgG2b |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 7812.5-500000 pg/ml |
| Sensitivity | 3906.25 pg/ml |
| Specificity | Do not react with Mouse IgG2b analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠IgG2b单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品或预稀释的样本和辣根过氧化物酶（HRP）标记的抗小鼠IgG2b抗体，标准品或样本和酶标抗体的复合物会与酶标板上的包被抗体结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠IgG2b，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠IgG2b浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠IgG2b的浓度。 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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