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My CartReactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：5.8 pg/ml
Storage：2-8℃，6 months
Gene ID：627
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| Name | Human BDNF ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | BDNF |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 11.71-750 pg/ml |
| Sensitivity | 5.8 pg/ml |
| Specificity | Do not react with Human CNTF，β-NGF，NGFR，NT-3，NT-4，TrkC， Mouse TrkC，TrkB，β-NGF，NGFR etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P23560 |
| Gene ID | 627 |
| Detection Principle | The Solarbio ELISA kit uses enzyme-linked immunosorbent assay technology based on the dual-antibody sandwich method. Anti-human BDNF monoclonal antibody was coated on a microplate label; Serial dilutions of standards and pre-diluted samples were added, respectively, and the human BDNF in the standard and sample would be well bound to the coated antibody on the plate; After washing, a biotinylated anti-human BDNF antibody is added, which binds specifically to the standard captured by the antibody-coated on the plate and the human BDNF in the sample; After washing, horseradish peroxidase (HRP)-labeled streptavidin was added, and biotin and streptavidin would undergo high-intensity non-covalent binding; If there are different concentrations of human BDNF in the sample in the reaction well, HRP will make the colorless TMB turn into a blue substance of different shades (positive correlation), and the reaction hole will turn yellow after adding the stop solution; Finally, the absorbance (OD) of the reaction well sample was measured at λmax=450nm (OD=450nm), and the concentration of human BDNF in the sample was proportional to the OD, and the concentration of human BDNF in the sample could be calculated by plotting the standard curve and four-parameter fitting software. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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