Human IL-6 ELISA Kit
Cat.No：SEKH-0013 Solarbio
Application： ELISA
Reactivity：Human
Sensitivity：2pg/mL
Storage：2-8℃，6 months
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Human IL-6 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-6 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Sensitivity | 2pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P05231 |
| Gene ID | 3569 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IL-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人IL-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人IL-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人IL-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人IL-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人IL-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人IL-6的浓度。 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no experimental images.
Related Products
Sorry, there is no more information.
Related Paper
Sentinel supervised lung-on-a-chip: A new environmental toxicology platform for nanoplastic-induced lung injury
Click to check >>Author：Sheng Yang, Tianyi Zhang, Yiling Ge, Yanping Cheng, Lihong Yin, Yuepu Pu, Zaozao Chen, Geyu Liang
IF：13.6000
Publish_to：JOURNAL OF HAZARDOUS MATERIALS
PMID：37406524
Near-infrared light?triggered platelet arsenal for combined photothermal-immunotherapy against cancer
Click to check >>Author：栗锋
IF：13.1160
Publish_to：
PMID：33771861
‘Green’ nanotherapeutics from tea leaves for orally targeted prevention and alleviation of colon diseases
Click to check >>Author：俎梦航
IF：12.4000
Publish_to：
PMID：34656857
Optical Microfiber Reader for Enzyme-Linked Immunosorbent Assay
Click to check >>Author：黄艳
IF：6.3500
Publish_to：
PMID：31599567
Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling
Click to check >>Author：卢从华
IF：6.2680
Publish_to：COMMUNICATIONS BIOLOGY
PMID：35197546
nanobodies as solubilization chaperones for the expression and purification of inclusion-body prone proteins
Click to check >>Author：姚光帅
IF：6.2200
Publish_to：chemical communications
PMID：35137750
Hsa_circ_0007707 participates in PDE3B-mediated apoptosis inhibition and inflammation promotion in fibroblast-like synoviocytes
Click to check >>Author：Yanhua Zuo, Huaheng Xu, Yanxia Li, Zongfang Zhang, Ran Tao, Manxiang Wang
IF：5.7140
Publish_to：INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：37086679
Metformin Combining PD-1 Inhibitor Enhanced Anti-Tumor Efficacy in STK11 Mutant Lung Cancer Through AXIN-1-Dependent Inhibition of STING Ubiquitination
Click to check >>Author：Wang Z
IF：5.2460
Publish_to：Frontiers in molecular bioscience
PMID：35281267
Coptisine inhibits Helicobacter pylori and reduces the expression of CagA to alleviate host inflammation in vitro and in vivo
Click to check >>Author：Qin Tang, Zhengcai Ma, Xiang Tang, Yan Liu, Huimin Wu, Yu Peng, Baihua Jiao, Rui Wang, Xiaoli Ye, Hang Ma, Xuegang Li
IF：5.1950
Publish_to：JOURNAL OF ETHNOPHARMACOLOGY
PMID：37164257
Haploinsufficiency of A20 caused by a novel pathogenic missense variant of TNFAIP3 and successfully treated with anti-TNF and immunosuppressive therapies
Click to check >>Author：Sun Lina, Han Yanan, Yang Ying, Wang Fengfan, Hou Xin, Ren Xiaoxia, Fang Ying
IF：4.3000
Publish_to：CELLULAR IMMUNOLOGY
PMID：37535999
Dabie bandavirus infection induces macrophagic pyroptosis and this process is attenuated by platelets
Click to check >>Author：Sicong Yu, Qinyi Zhang, Lingxuan Su, Ji He, Wen Shi, Hao Yan, Haiyan Mao, Yi Sun, Dongqing Cheng, Xuan Wang, Yanjun Zhang, Lei Fang
IF：3.8000
Publish_to：PLoS Neglected Tropical Diseases
PMID：37486928
Structural and biofunctional evaluation of decellularized jellyfish matrices
Click to check >>Author：Wen-Bo Wu, Xiao-Fei Jiang, Ming-Quan Chen
IF：3.3000
Publish_to：KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
PMID：37306210
Interference of periostin attenuates pathological changes,proinflammatory markers and renal fibrosis in diabetic kidney injury
Click to check >>Author：Duan Xiaoting, Chen Cheng, Liu Xiaoli, Wang Taoxia, Feng Shuning, Li Jianwei, Li Guiying
IF：2.1640
Publish_to：Genes & Genomics
PMID：37248423
Technical Q&A
Q&A
（Total 0
User Q&A）
- There is not question and answer now, come and send an inquiry please.
My question
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: [email protected]
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.