L-Glutamine，Liquid(200mM)

Phalloidin is a kind of cyclic heptapeptide toxin derived from amanita phalloides. It selectively binds to filamentous actin F-actin with high affinity (Kd=20 nM), but does not bind to monomer actin G-actin. It is usually used to label F-actin in tissue sections, cell cultures or cell-free systems, so as to qualitatively and quantitatively analyze F-actin. In addition, phalloidin derivatives also bind to large and small fibers with similar affinity, whether they are muscle cells of animal or plant origin or non-muscle cells, according to the stoichiometric ratio of each actin subunit to one phalloidin molecule. And the non-specific binding is almost negligible, and the recognition of stained and non-stained regions is very obvious. Therefore, cyclopeptide derivatives are especially suitable to replace actin (Actin) antibodies in related research. In addition, the derivatives of phalloidin are very small, with a diameter of about 12-15?, and molecular weight < 2000 Daltons. Many physiological properties of unlabeled actin (Actin) can be maintained, for example, they can still react with actin binding proteins such as myosin, promyosin, DNaseI; the filaments labeled with phalloidin can still penetrate the solid phase myosin matrix; and glycerol-extracted muscle fibers can still contract after labeling.

The binding of phalloidin prevents the dissociation of filamentous actin (microfilament) and stabilizes the structure of microfilament, thus destroying the dynamic balance of polymerization-depolymerization of microfilament. This property reduces the critical concentration (CC) of actin polymerization to < 1 μg/mL, so it can be used as a polymerization accelerator. In addition, Phalloidin can also inhibit the ATP hydrolysis activity of F-actin.

This product is a FITC-labeled Phalloidin with strong specificity and high contrast. It has a better staining effect than Actin antibody and is suitable for qualitative and quantitative detection of F-actin. In addition, the F-actin combined with this product can still maintain many biological characteristics of actin itself. And the combination of this product has no species difference and has a wide range of applicability.

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

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