Thiolglycollate Medium
Cat.No：LA0561 Solarbio
Storage：RT
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Oscillating Mixing & Stirring & Dispersing Instrument
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Product Information
| Name | Thiolglycollate Medium |
| Storage | RT |
| Grade | BR |
| Unit | Bottle |
| Specification | 250g |
Product introduction
Application of aerobic bacteria sterility test in turbid products
Principle: casitone and yeast extract powder provide carbon and nitrogen sources, vitamins and growth factors; Glucose provides fermentable sugars that are more conducive to growth; Sodium chloride was used to maintain the equilibrium osmotic pressure. Sodium thioethanolate and L-cystine can effectively reduce the REDOX potential and prevent the accumulation of peroxides from being toxic to some bacteria. At the same time, their sulfhydryl groups can passivate the antibacterial effect of preservative containing arsenic, mercury and other heavy metals. The coagulation of a small amount of Agar prevents the diffusion of carbon dioxide, oxygen and reduction products; Resazurin is a REDOX indicator that is pink in the oxidation state and colorless in the reduction state.
preparation method
Weigh 29.3 grams of this product, add it to 1000ml distilled water, heat and boil the solution, divide into aliquots, autoclaving at 121℃ for 15 minutes and set to use.
composition: g/L
casitone: 15
Yeast extract powder: 5
Glucose: 5
Sodium thioglycolate: 0.5
Sodium chloride: 2.5
L-cystine: 0.5
Agar: 0.75
Resazurin: 0.001
pH value: 7.1±0.2
Precautions
Do not use this product if clumping occurs. After sterilization, the product should be operated in a sterile environment.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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