RPF Agar Medium Base
Cat.No：LA3770 Solarbio
Storage：RT，keep dry
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Oscillating Mixing & Stirring & Dispersing Instrument
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Product Information
| Name | RPF Agar Medium Base |
| Storage | RT，keep dry |
| Grade | BR |
| Unit | Bottle |
| Specification | 250g |
Product Introduction:
It is used for the detection of coagulase positive staphylococci.
Preparation Method:
Weigh 58g of this product in 900ml distilled water, heat and boil until completely dissolved, subpack, autoclave at 121℃ for 15 minutes, shake well after sterilization to prevent Agar from settling on the bottom of the vessel and coagulating, cool to 50℃. In aseptic procedure, sterile potassium tellurite 2.5mg, rabbit plasma 2.5ml, bovine fibrinogen solution 7.5ml and pancreatic enzyme inhibitor 2.5ml were added into every 100ml medium, and the pour plate was gently shaken. (1) Bovine fibrinogen solution: Take 5g bovine fibrinogen and dissolve it in 100ml distilled water,0.22um sterile filter membrane to filter and remove bacteria. (2) Pancreatic enzyme inhibitor: 30mg of pancreatic enzyme inhibitor was taken into 30ml distilled water, and 0.22um sterile filter membrane was used to filter and remove bacteria.
Composition: g/L
Casein peptone: 10.0
Beef powder: 5.0
Yeast powder: 1.0
Sodium pyruvate: 10.0
L-glycine: 12.0
Lithium chloride: 5.0
Agar: 15.0
pH: 7.2±0.2, 25℃
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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