Thiosulfate Citrate Bile Salts Sucrose Agar
Cat.No：LA3170 Solarbio
Storage：RT，3 years
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> Microbial Culture > Other Microbial Medium > Thiosulfate Citrate Bile Salts Sucrose Agar
Product Information
| Name | Thiosulfate Citrate Bile Salts Sucrose Agar |
| Storage | RT，3 years |
| Grade | BR |
| Unit | Bottle |
| Specification | 250g |
Product introduction
For the selective isolation and culture of Vibrio cholerae and other enteric pathogenic vibrio SPP.
Principle
peptone provides nitrogen, carbon, vitamins and other nutrients for growth as a basic nutrient; Sucrose as a fermentable carbohydrate; Sodium chloride could stimulate the growth of Vibrio SPP. Sodium cholate, beef bile powder, sodium thiosulfate, sodium citrate and higher pH could inhibit Gram-positive bacteria and coliform bacteria. Vibrio cholerae was sensitive to acidic environment, and its growth was enhanced by high pH value. The reaction of sodium thiosulfate with ferric citrate was used as an indicator to detect the production of hydrogen sulfide. Bromothymol blue and thymol blue were used as acid-base indicators.
preparation method
Weigh 89 grams of this product, add to 1000ml of distilled water, dissolve by boiling, sterilize, and pour into the sterilization plate when cold to 50-55℃. Spare.
composition: g/L
Polyvalent peptone: 10
Sodium cholate: 3
Sodium citrate: 10
Bovine bile powder: 5
Bromothymol blue: 0.04
Agar: 15
Sodium chloride: 10
Ferric citrate: 1
Yeast extract powder: 5
Sodium thiosulfate: 10
Thymol blue: 0.04
Sucrose: 20
pH: 8.6±0.2
Precautions
This product is not high pressure, sterilization after operation in a sterile environment.
Do not use this product if clumping occurs.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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