X-gal(20mg/ml)
Cat.No:X1010 Solarbio
Storage:Store at -20℃,avoid light,1 year.
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Name | X-gal(20mg/ml) |
Storage | Store at -20℃,avoid light,1 year. |
Unit | Bottle |
Specification | 1ml 5ml |
Product Description:
IPTG was used as a placebo inducer and X-gal as a chromogenic substrate, and both were used for blue-white spot screening. IPTG can induce the synthesis of an amino-terminal fragment of β-galactosidase from the Lac operon DNA segment of the vector, which can achieve intragenic complementation (α-complementation) with the defective β-galactosidase encoded by the host cell. Bacteria achieving alpha complementation spread on medium containing X-gal chromogenic substrate formed blue colonies. The insertion of exogenous DNA into the polyclonal site of the plasmid can destroy the α complementation and produce white colonies.
This product is a non-sterile solution of X-gal dissolving with DMF.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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