Fluo-3,AM *5mM solution in anhydrous DMSO*
Cat.No:F8841 Solarbio
Storage:Store at -20℃,avoid light,1 year
CAS:121714-22-5
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My CartStorage:Store at -20℃,avoid light,1 year
CAS:121714-22-5
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Size:
Name | Fluo-3,AM *5mM solution in anhydrous DMSO* |
CAS | 121714-22-5 |
Molecular Formula | C51H50Cl2N2O23 |
Molecular Weight | 1129.85 |
Purity/Grade | ≥90% |
Solubility | Soluble in DMSO |
Ex/Em(nm/nm) | 506nm/526nm |
Storage | Store at -20℃,avoid light,1 year |
Unit | Bottle |
Specification | 20uL |
Product introduction:
Fluo-3, AM is one of the most commonly used fluorescent probes for the detection of intracellular calcium concentration. After it penetrates the cell membrane and enters the cell, it is cleaved by intracellular esterase to form Fluo-3, which is retained in the cell. Fluo-3 is almost non-fluorescent when it exists in the form of free ligand, but when it combines with intracellular calcium ions, it can produce strong fluorescence with a maximum excitation wavelength of 506nm and a maximum emission wavelength of 526nm. The recommended excitation wavelength is about 488nm, and the emission wavelength is 525-530nm. The changes of intracellular calcium ion concentration can be detected by laser confocal microscopy or flow cytometry.
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Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Click to check >>Author:Yunzhao Chen, Dandan Wang, Hao Peng, Xi Chen, Xuep
IF:7.7760
Publish_to:Molecular Cancer
PMID:30609930