Fluo-4，AM
Cat.No：F8500 Solarbio
Appearance：Orange Lyophilized Powder
Storage：Store at -20℃，avoid light and keep dry，1 year
CAS：273221-67-3
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HIF
Immunology & Inflammation
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
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Product Information
| Name | Fluo-4，AM |
| CAS | 273221-67-3 |
| Molecular Formula | C51H50F2N2O23 |
| Molecular Weight | 1096.94 |
| Appearance | Orange Lyophilized Powder |
| Ex/Em(nm/nm) | 494nm/516nm |
| Storage | Store at -20℃，avoid light and keep dry，1 year |
| Unit | Bottle |
| Specification | 100ug 1mg 10*100ug |
Product introduction:
Fluo-4 is a calcium fluorescent probe that replaces Cl with F in the Fluo-3 structure. By replacing Cl with F, which is more attractive to electrons, its maximum excitation wavelength will deviate about 10 nm to the short wavelength. This wavelength is closer to that of the argon laser, so Fluo-4 is twice as strong as Fluo-3 when excited with an argon laser.
Fluo-4, AM penetrates the cell membrane into the cell and is cleaved by intracellular esterase to form Fluo-4, which is trapped in the cell. Fluo-4 is almost non-fluorescent when it exists as a free ligand, but can produce strong fluorescence when it binds to intracellular calcium ions. The maximum excitation wavelength is 494nm, and the maximum emission wavelength is 516nm. The changes of intracellular calcium ion concentration can be detected by laser confocal microscopy or flow cytometry.
HBSS: no calcium, no magnesium, no phenol red
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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