Calcein，AM
Cat.No：C8950 Solarbio
Appearance：White or Light Yellow Powder
Storage：Store at -20℃，avoid light and keep dry，1 year
CAS：148504-34-1
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | Calcein，AM |
| CAS | 148504-34-1 |
| Molecular Formula | C46H46N2O23 |
| Molecular Weight | 994.86 |
| Purity/Grade | ≥90% |
| Appearance | White or Light Yellow Powder |
| Storage | Store at -20℃，avoid light and keep dry，1 year |
| Unit | Bottle |
| Specification | 1mg |
Product Introduction：
Calcein, AM is a cell staining reagent that can fluorescent label living cells. After it penetrates the cell membrane and enters the cell, it is cleaved by intracellular esterase to form Calcein, which is retained in the cell and emits strong green fluorescence. Compared with other similar reagents such as BCECF, AM and CFDA, Calcein, AM showed very low cytotoxicity. The excitation and emission wavelengths of Calcein are 490 nm and 515 nm, respectively.
Calcein, AM stained only viable cells. As a nuclear dye, PI cannot cross the cell membrane of living cells. It passes through the disordered region of the dead cell membrane and reaches the nucleus, where it intercalates into the DNA double helix of the cell to produce red fluorescence (excitation: 535 nm, emission: 617 nm), so PI only stains dead cells. Since both Calcein and PI-DNA can be excited by 490 nm, both live and dead cells can be visualized by fluorescence microscopy. Only dead cells are observed with excitation at 545 nm.Calcein, AM and PI are often combined to double stain live and dead cells which are based on above characteristics.
Since the optimal staining conditions differ between cell lines, we recommend individually determining the appropriate concentrations of Calcein, AM,and PI.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no more information.
Related Products
Sorry, there is no more information.
Related Paper
Bionic natural small molecule co-assemblies towards targeted and synergistic Chemo/PDT/CDT
Click to check >>Author：Fu Shiyao, Wang Mingao, Li Bin, Li Xu, Cheng Jianjun, Zhao Haitian, Zhang Hua, Dong Aijun, Lu Weihong, Yang Xin
IF：15.8630
Publish_to：Biomaterials Research
PMID：37161611
A PDA-Functionalized 3D Lung Scaffold Bioplatform to Construct Complicated Breast Tumor Microenvironment for Anticancer Drug Screening and Immunotherapy
Click to check >>Author：Wanheng Zhang, Yan Chen, Mengyuan Li, Shucheng Cao, Nana Wang, Yingjian Zhang, Yongtao Wang
IF：15.1000
Publish_to：Advanced Science
PMID：37424037
Microvascularized tumor assembloids model for drug delivery evaluation in colorectal cancer-derived peritoneal metastasis
Click to check >>Author：Qijun Lv, Yizhen Wang, Zhiyong Xiong, Yifan Xue, Jiajun Li, Moyang Chen, Kaijian Zhou, Hetao Xu, Xiaoge Zhang, Jie Liu, Jie Ren, Bo Liu
IF：9.7000
Publish_to：Acta Biomaterialia
PMID：37393969
Tumor Cell Targeting and Responsive Nanoplatform for Multimodal-Imaging Guided Chemodynamic/Photodynamic/Photothermal Therapy toward Triple Negative Breast Cancer
Click to check >>Author：Lihong Li, Jiaojiao Li, Rongrong Hu, Xinyu Zhang, Lei Ding, Guodong Ren, Wen Liu, Haojiang Wang, Bin Wang, Chengwu Zhang, Haipeng Diao
IF：9.5000
Publish_to：ACS Applied Materials & Interfaces
PMID：37261936
Precise quantification of the antibacterial activity of chitosan by NB medium neutralizer
Click to check >>Author：王梦阳
IF：6.1550
Publish_to：
PMID：
Zn2+-interference and H2S-mediated gas therapy based on ZnS-tannic acid nanoparticles synergistic enhancement of cell apoptosis for specific treatment of prostate cancer
Click to check >>Author：Bo Zhou, Ben-Xu Jia, Ming-Jin Zhang, Yan-Jun Tan, Wei-Yuan Liang, Xiang Gan, Hong-Tao Li, Xiaoli Yang, Xing-Can Shen
IF：5.9990
Publish_to：COLLOIDS AND SURFACES B-BIOINTERFACES
PMID：37075522
Red blood cell membrane-functionalized Nanofibrous tubes for small-diameter vascular grafts
Click to check >>Author：Yue Jiang, Shanzhu Guo, Jingjing Jiao, Long Li
IF：5.0000
Publish_to：Polymers
PMID：37376390
U-Shaped Conjugated Oligomer-Based Micelles as a Ratiometric Fluorescent Nanothermometer for Diverse Cellular Applications
Click to check >>Author：Liu Xi, Zhao Yu, Niu Yangyang, Xie Qionghong, Nie Hao, Jin Yun, Zhang Yingying, Lu Yuqiu, Zhu Saiya, Zuo Wei, Yu Chen
IF：4.6000
Publish_to：Clinical Kidney Journal
PMID：
Guluronic acid can inhibit copper(II) and amyloid???β peptide coordination and reduce copper-related reactive oxygen species formation associated with Alzheimers disease
Click to check >>Author：Hangyu Shen, Yun Dou, Xiaoying Wang, Xiaohui Wang, Fangong Kong, Shoujuan Wang
IF：4.3360
Publish_to：JOURNAL OF INORGANIC BIOCHEMISTRY
PMID：37207465
Technical Q&A
Q&A
（Total 0
User Q&A）
- There is not question and answer now, come and send an inquiry please.
My question
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: [email protected]
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.