Trypsin
Cat.No：T8151 Solarbio
CAS：9002-07-07
Storage：Store at 2-8℃
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Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
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MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| CAS | 9002-07-07 |
| Storage | Store at 2-8℃ |
| Enzyme Activity/Titer | 4000U/g |
| Unit | Bottle |
| Specification | 500g |
Product introduction
This product is a mixture of enzymes extracted and refined from the pancreas of pig, cow or sheep. It mainly contains protease, pancreatic amylase and pancreatic lipase. This product is widely used in proteolysis, peptide and amino acid production.
Usage method
Usually in the decomposition of proteins, the temperature is controlled at 45-55 ° C, the initial pH is adjusted to about 8.0, and the pH between 7.5 and 8.5 should be adjusted frequently as the hydrolysis proceeds (because the decomposition of proteins will produce amino acids, which will decrease the pH and affect the enzyme activity) until the end of the hydrolysis.
Unit definition
Ultraviolet spectrophotometry: under the measurement conditions (40±0.2℃; PH 8.0). When the absorbance of TCA-soluble substance released from casein hydrolysis per minute at 275nm is equivalent to that of 1μmol tyrosine, the amount of enzyme required is one unit of activity, which is expressed as u/g.
Optimum pH
8
pH Stability
7.5-8.5
Optimum temperature
45℃
Thermal stability
40~50 ℃
Matters needing attention
1. This product is only used in the field of scientific research, not edible or used in food production.
2. Inhalation of enzyme dust and enzyme fog should be avoided. If accidentally splashed on hands and eyes, should immediately rinse with water for more than 15 minutes.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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