β- Glucosidase

Product introduction

β-glucosidase belongs to the cellulases, also known as cellobiose enzymes, originated from Aspergillus Niger. It can act on cellobiose or cellooligosaccharides to assist exo-glucanase and endoglucanase to completely hydrolyze cellulose molecules to glucose. In addition, β-glucosidase can also act on the glycosidic bond between the sugar group and the aryl group or hydrocarbon group, so that the corresponding glycoside compounds are degraded to generate ligand and glucose.

Usage method

1. Conditions of use:

The temperature was 40-55 ° C, and the optimal temperature was 45-50 ° C

PH 4.5-6.0, optimal PH 4.5-5.0

2, recommended dosage: 0.3-0.5% (substrate dry weight)

3. Usage: the substrate is immersed in heated water for 30min, and then activated by 40℃ water for 5-10min. When the temperature and PH value are adjusted to the required conditions, the enzyme solution is slowly added and gently stirred. The constant temperature enzymatic hydrolysis is about 2h (according to the specific substrate), and the extraction process is carried out after the enzymatic hydrolysis.

Unit definition

The amount of enzyme hydrolyzing 1% of barley β-glucan to yield 1μM glucose per minute at 40 ° C, pH6.0 was defined as one unit of enzyme activity.

Matters needing attention

Safety performance:

Avoid inhalation, eyes contact this product, should immediately wash with water.

Transportation and storage:

This product is an active biological agent. During transportation and storage, it should be protected from light, low temperature, dry and ventilated. The shelf life is 18 months.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.