α-Amylase

Product introduction

A-amylase, also known as A-1, 4-glucan-4-glucan hydrolase, is a starch endonuclease hydrolase with starch as substrate. When it interacts with starch, it cuts the A-1, 4-glucoside bond from the inside of the molecule to rapidly reduce the molecular weight of starch. A-amylase cannot cut the A-1, 6-glucoside bond at the branch point of amylopectin. The A-1, 4-glucoside bond near the A-1, 6-glucoside bond cannot be cut. Thus, in addition to glucose and maltose, the hydrolysate also contains dextrins and a series of oligosaccharides containing A-1, 6-glucoside bonds. Enzyme activity: 10000U/g, this product is fermented by Bacillus, with high enzyme activity, good heat resistance, wide pH range, no additives, no toxic side effects and so on. The effect of calcium ions on enzyme activity: calcium ions can improve the stability of enzyme activity, without calcium ions, enzyme activity is completely lost.

Scope of application

Suitable for liquefaction processes up to 90 ° C

Usage method

The recommended dosage is 6-8U/g starch, and the specific amount should be determined according to the actual process conditions. After conversion, it is recommended to add 1kg starch and 0.6-0.8g enzyme powder.

Unit definition

Above 70℃, pH6.0, 1 minute to liquefy soluble starch 1 mg to dextrin required enzyme amount is 1 enzyme activity unit, expressed in u/ml

recommended name

EC 3.2.1.1

Optimum pH

6.0 (stable pH range6.0-7.0, pH5.0 severe inactivation)

Optimum temperature

60-70℃

Thermal stability

Stable under 60 ℃

Inhibitors

Copper, titanium, cobalt and other metal ions have a certain impact on this product. Lead, aluminum, zinc and other metal ions on the product has a strong inhibitory effect.

Matters needing attention

As the bioactive substance of this strain, strong light and high temperature will cause enzyme inactivation, it is recommended to store it sealed and in a dry and low temperature environment, below 25℃, for 12 months in liquid storage.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.