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Application：ELISA
Reactivity：Human
Sample Type：Serum, plasma, Cell culture supernatant
Sensitivity：125 pg/ml
Storage：2-8℃,6 months
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| Name | Human Pro-Collagen II ELISA Kit |
| Storage | 2-8℃,6 months |
| Detection Target | Pro-Collagen II |
| Sample Type | Serum, plasma, Cell culture supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 250-16000 pg/ml |
| Sensitivity | 125 pg/ml |
| Specificity | Do not react with human BMP-1，Pro-Collagen Iα1，Pro-Collagen IIIα1，Collagen XXIIIα1 etc. |
| Precision | Coefficient of variation is < 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Detection Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Pro-Collagen II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Pro-Collagen II present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Pro-Collagen II is added to detect the captured Pro-Collagen II protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm. |
| Unit | Box |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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