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My Cart> Biochemical Assay Kits > Fatty Acid Metabolism Series > Total bile acid (TBA) Content Assay Kit (Enzymatic Cycling Method)
Detection Method：Spectrophotometer
Storage：Store at -20℃，6months
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| Name | Total bile acid (TBA) Content Assay Kit (Enzymatic Cycling Method) |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6months |
| Unit | kit |
| Specification | 50T/48S |
Total bile acid (TBA) is the product of cholesterol catabolism in the liver and is the largest organic acid secreted by the liver into bile. When liver cell injury or biliary obstruction, it will cause bile acid metabolism disorder and increase serum bile acid. Therefore, the determination of bile acid can be used as an important information to judge whether the hepatobiliary system is normal or not.
Bile acids are catalyzed by 3α-hydroxysteroid dehydrogenase ( 3α-HSD ) to generate 3-oxocholic acid and NADH, which react with nitroblue tetrazolium iodide ( INT ) to form a pink substance with a maximum absorption peak at 500 nm. Based on this, the content of total bile acids can be calculated.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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