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My Cart> Biochemical Assay Kits > Fatty Acid Metabolism Series > Glycerol Kinase (GK) Activity Assay Kit
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6months
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| Name | Glycerol Kinase (GK) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6months |
| Unit | kit |
| Specification | 100T/96S |
Glycerol kinase (GK, EC 2.7.1.30 ) is a key enzyme in the pathway of glucose metabolism. It is responsible for the uptake and metabolism of glycerol in vivo. It catalyzes the phosphorylation of glycerol, which is the first step in the glucose metabolism pathway. By measuring the activity of glycerol kinase, we can understand the status of glucose metabolism, which is helpful for the diagnosis and treatment of diseases.
Glycerol is oxidized to glycerol phosphate by glycerol kinase (GK), and glycerol phosphate oxidase (GPO) oxidizes glycerol phosphate to produce hydrogen peroxide. Hydrogen peroxide reacts with 4-aminoantipyrine and 2,4-dichlorophenol to form a red product, which has a characteristic absorption peak at 505 nm. According to this, the activity of glycerol kinase can be calculated.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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