Jurkat, Clone E6-1 人T淋巴细胞白血病细胞
Cat.No：SCC-121815 Solarbio
Storage：Store at -70℃，avoid freeze/thaw cycle.
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Oscillating Mixing & Stirring & Dispersing Instrument
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Product Information
| Name | Jurkat，Clone E6-1 |
| Storage | Store at -70℃，avoid freeze/thaw cycle. |
| Transport Condition | The product is shipped on dry ice pack. |
| Culture Condition | RPMI-1640＋10% FBS＋1% P/S |
| Cell Type | Suspension cell |
| Background | Jurkat, Clone E6-1 cell is a clone of the Jurkat-FHCRC cell line (derived from the Jurkat cell line).Jurkat, Clone E6-1 cells, derived from the peripheral blood of a 14-year-old boy, produced large amounts of IL-2 after induction with phorboate and lectins or anti-T3 monoclonal antibodies (both types of induction are required). |
| Source | T lymphocytes; Acute T-cell leukemia |
| Note | The cells are suspension cells, please pay attention to centrifuge to collect cell suspension; Do not throw away the cell culture solution directly. |
| Unit | Piece |
| Specification | 1EA |
贴壁细胞培养
细胞名称：人T淋巴细胞白血病细胞；Jurkat, Clone E6-1
形态特性：淋巴母细胞样
生长特性：悬浮生长
培养条件：RPMI 1640 (w/o Hepes) + 10%FBS
传代方法：维持细胞浓度在1×105~1×106 cells/ml；2~3天换液1次。
冻存条件：基础培养基+8%DMSO+20%FBS
特征特性： 该细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆。Jurkat细胞株来源于一个14岁男孩的外周血；经佛波酯和外源凝集素或抗T3单克隆抗体中的一种诱导后可产生大量IL-2(IL-2的产生需两种类型的诱导剂)；表达T细胞受体、CD3。
细胞处理方法：
1. 细胞在培养瓶中培养至状态良好后灌满培养基运输，获得细胞后用酒精棉球擦拭瓶口消毒，然后在超净台中操作。
2. 将细胞转移至50mL的无菌离心管中，1000rpm离心5-10min，用完全培养基重悬细胞，适宜的密度分瓶培养。
特别注意：（如使用公共实验室或初次接触细胞培养，建议添加双抗培养）
1. 我们使用自产培养基及进口血清培养细胞，在您拿回细胞后，如想更换其它品牌培养基， 请依照逐次替换的原则，先保留培养瓶中的培养基，多日多次代逐步更换，以减轻对细胞的刺激。
2. 如签收时出现培养瓶壁破裂，漏液等情况请及时拍照并联系售后。
3. 细胞任何售后问题，均需拍照存档并在2周之内及时联系客服。
相关产品及货号：
RPMI 1640 (31800)
细胞冻存液（24800）
1×PBS缓冲液(pH7.2- 7.4)（P1020）
0.25%胰蛋白酶-EDTA消化液（T1300）
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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