Uracil DNA Glycosidase (UNG Enzyme/UDG Enzyme)

Product introduction

Uracil DNA glycosidase (UNG, UDG) is a 25kDa enzyme that catalyzes the release of free uracil from single-stranded and double-stranded DNA containing uracil.

Scope of application

The UNG enzyme has no activity against RNA and is mainly used to prevent contamination of PCR amplified products.

Unit definition

The amount of enzyme used to degrade 1μg of single-stranded DNA containing dU bases for 1 hour at 37 ° C was 1 unit.

Optimum pH

8

Optimum temperature

25℃

Matters needing attention

1, avoid multiple freezing and thawing, do not be exposed to large temperature fluctuations. Temperature fluctuation has a great influence on the stability of the product.

2. UNG enzyme can remove accidentally contaminated PCR products containing dUTP before PCR reaction, thus avoiding false positive results caused by contamination;

3. Because different genes to be amplified have different utilization efficiency of dUTP and sensitivity to UNG enzyme, the reaction system should be adjusted and optimized if the detection sensitivity is decreased due to the use of UNG system.

4. Use special areas and pipettes before and after amplification, wear gloves to operate and change frequently, and do not open the reaction tube after the PCR reaction is completed. To minimize the contamination of samples by PCR products.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.