Protease Inhibitor Mixture
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Cat.No：P6730 Solarbio
Appearance：Store at -20℃，avoid light，1 year.
CAS：Liquid
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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MAPK
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Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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> Protein and Immunity > Protein Extraction/Quantification > Protein Extraction Reagent > Protease Inhibitor Mixture
Product Information
| Gene Name | Piece |
| CAS | Liquid |
| Appearance | Store at -20℃，avoid light，1 year. |
| Specification | 1ml |
Product introduction
Protease Inhibitor (Protease Inhibitor) is a substance that binds with some groups on the active center of the protease molecule to reduce or even disappear the activity of the protease but does not denature the enzyme protein. There are many kinds of protease inhibitors, including EDTA, E-64, NaVO3, Bestatin, Leupetin, PepstatinA, Aprotinin, etc., which can effectively inhibit protein degradation and maintain the original protein-protein interaction. Protease inhibitor mixture (100×PIC) was mainly composed of Leupetin, PepstatinA, Aprotinin, E-64, etc， and did not contain EDTA. The mixture can inhibit the activity of most proteases, including serine protease, cysteine protease, pepsin, trypsin, papain and so on. The protease inhibitor mixture is a 100× concentrated DMSO solution, which is suitable for extracting protein from mammalian tissues and cells, and can obtain the target protein more effectively.
Scope of application
The extracted protein can be used for Western Blot, immunoprecipitation and other tests.
Usage method
1. Before opening the cover, please centrifuge at low speed, so as to throw the liquid adhering to the tube wall to the bottom of the tube.
2. When used, protease inhibitor mixture (100×PIC) was added according to the amount of lysate, so that the final concentration reached 1×.
Notes:
1, protease inhibitor mixture has slight irritation, careful operation, and should avoid repeated freezing and thawing.
2, for your safety and health, please wear a lab coat and wear disposable gloves to operate.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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稀释后保存在冰箱会影响抑制剂功能吗？稀释后可以使用多久呢 2023-05-29 14:58:27
Administrator您好，蛋白酶抑制剂使用时,根据裂解液的用量加入蛋白酶抑制剂混合液(100×PIC)，使其终浓度达到1×，直接用于提蛋白，不建议提前稀释。
您好，PIC可以抑制磷酸化蛋白去磷酸化吗？ 2022-09-20 14:08:49
Administrator不能够抑制磷酸酶活性，抑制磷酸化蛋白去磷酸化需再添加磷酸酶抑制剂
需要和PMSF合用，还是单用即可？ 2022-08-01 11:02:31
Administrator您好，需要同时加PMSF的，按照浓度比例要求添加到蛋白裂解液中使用