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My Cart> Biochemical Assay Kits > REDOX Series > Oxidation Series > Serum (Plasma) Diamine Oxidase (DAO) Activity Assay Kit
Storage：Store at 2-8℃，6months
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| Name | Serum (Plasma) Diamine Oxidase (DAO) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6months |
| Unit | kit |
| Specification | 100T/48S |
DAO (Diamine oxidase,EC1.4.3.6) is widely present in animals (intestinal mucosa,lung,liver,kidney,etc. ),plants and microorganisms.It catalyzes the oxidation of polyamines to aldehydes, and its activity is closely related to the synthesis of nucleic acids and proteins, which can reflect the integrity and damage of the intestinal mechanical barrier.
DAO catalyzes cadaverine to produce aldehyde and hydrogen peroxide. H2O2 oxidizes Fe2+ in potassium ferrocyanide to form Fe3+. Fe3+ further reacts with 4-aminoantipyrine and phenol to form red quinone compounds. There is a characteristic absorption peak at 505 nm. The activity of DAO is calculated by measuring the absorbance at 505 nm.
The kit is only suitable for serum (plasma) samples, other samples please use BC1280/BC1285.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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